Deep partial thickness burn blister fluid promotes neovascularization in the early stage of burn wound healing.

The effect of burn blister fluid in neovascularization during burn wound healing is unknown. Burn blister fluid, containing a large amount of chemokines, is thought to play a role in the early stage of neovascularization. This process includes angiogenesis and vasculogenesis. Because of different he...

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Publicado en:Wound Repair & Regeneration Vol. 18; no. 3; pp. 311 - 319
Autores principales: Pan S, Wu L, Chen C, Shieh S, Chiu H
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell May/Jun2010
Acceso en línea:Ver este registro en EBSCOhost
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      dt: May/Jun2010
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        atl: Deep partial thickness burn blister fluid promotes neovascularization in the early stage of burn wound healing.
      aug:
        au:
          Pan S
          Wu L
          Chen C
          Shieh S
          Chiu H
        affil: Institute of Molecular Medicine and Cardiovascular Research Center, College of Medicine, National Cheng Kung University, Tainan, Taiwan
      sug:
        subj:
          Blister
          Burns Physiopathology
          Fluids and Secretions
          Neovascularization, Physiologic
          Wound Healing Physiology
          Adolescence
          Adult
          Aged
          Chemokines
          Child
          Child, Preschool
          Descriptive Statistics
          Funding Source
          Human
          Immunohistochemistry
          In Vitro Studies
          Middle Age
          One-Way Analysis of Variance
          Polymerase Chain Reaction
          Adolescent: 13-18 years
          Adult: 19-44 years
          Aged: 65+ years
          Child: 6-12 years
          Child, Preschool: 2-5 years
          Middle Aged: 45-64 years
      ab: The effect of burn blister fluid in neovascularization during burn wound healing is unknown. Burn blister fluid, containing a large amount of chemokines, is thought to play a role in the early stage of neovascularization. This process includes angiogenesis and vasculogenesis. Because of different healing time of burn wounds, we hypothesized that neovascularization in superficial partial thickness burn (SPTB) and deep partial thickness burn (DPTB) wounds were different. The neovasculogenic effects of two different burn blister fluids were also different. We found Day 7 DPTB wounds had a significant increase in blood vessels compared with SPTB wounds by immunohistochemistry. DPTB blister fluid significantly promoted neovascularization via increasing endothelial cell proliferation, and migration and differentiation of circulating angiogenic cells relative to SPTB blister fluids. In the animal study, DPTB blister fluids markedly promoted new blood vessel formation compared with those from SPTB blister fluids using in vivo Matrigel plug assay. These results suggest that DPTB wounds require more new vessel formation than SPTB. Furthermore, the measurement of angiogenic activities in burn blister fluids serves as a possible tool for assessing burn wound status.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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