Distribution of Helicobacter pylori virulence markers in patients with gastroduodenal diseases in Pakistan.
Background: Helicobacter pylori (H. pylori) infection is known to be associated with a spectrum of gastroduodenal diseases. We studied the association of H. pylori virulence markers cytotoxin-associated gene (cagA) and vacuolating associated cytotoxin gene (vacA) alleles in patients with non ulcer d...
| Publicado en: | BMC Gastroenterology Vol. 9; pp. 87 - 88 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105258181&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105258181 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1471230X 1CHO jtl: BMC Gastroenterology issn: 1471230X maglogo: N pubinfo: dt: 2009 vid: 9 pid: 24147 pub: BioMed Central artinfo: ui: 105258181 NLM19930551 2010496667 10.1186/1471-230X-9-87 NLM19930551 PMC2784790 105258181 ppf: 87 ppct: 1 formats: tig: atl: Distribution of Helicobacter pylori virulence markers in patients with gastroduodenal diseases in Pakistan. aug: au: Yakoob J Abid S Abbas Z Jafri W Ahmad Z Ahmed R Islam M Yakoob, Javed Abid, Shahab Abbas, Zaigham Jafri, Wasim Ahmad, Zubair Ahmed, Rashida Islam, Muhammad affil: Department of Medicine, Aga Khan University, Karachi, Pakistan sug: subj: Biological Markers Analysis Gastrointestinal Diseases Epidemiology Helicobacter Infections Epidemiology Helicobacter Pylori Adolescence Adult Aged Aged, 80 and Over Alleles Antigens, Bacterial Bacterial Proteins DNA Female Gastrointestinal Diseases Metabolism Gastrointestinal Diseases Microbiology Genes Genotype Helicobacter Infections Metabolism Helicobacter Infections Microbiology Human Male Middle Age Morbidity Pakistan Polymerase Chain Reaction Retrospective Design Virulence Young Adult Adolescent: 13-18 years Adult: 19-44 years Aged: 65+ years Aged, 80 & over Middle Aged: 45-64 years Female Male ab: Background: Helicobacter pylori (H. pylori) infection is known to be associated with a spectrum of gastroduodenal diseases. We studied the association of H. pylori virulence markers cytotoxin-associated gene (cagA) and vacuolating associated cytotoxin gene (vacA) alleles in patients with non ulcer dyspepsia (NUD), gastric ulcer (GU), gastric carcinoma (GC) and duodenal ulcer (DU).Methods: H. pylori infection established by both rapid urease test and histology were studied. The cagA and vacA allelic status was determined by polymerase chain reaction (PCR). Sequencing of vacA i1 and i2 PCR product was carried out.Results: Two hundred and twenty-four patients were included, 141 (63%) were males with a mean age of 45 +/- 16, range 16-83 years. The virulence marker cagA was associated with GU in 20(63%) (p = 0.04), DU in 23(72%) (p = 0.003) and GC in 29(73%) (p = 0.001) compared to NUD in 51(42%). VacA s1am1 was associated with GU in 23(72%) (p = 0.001), DU in 17(53%) (p < 0.001) and GC in 23(58%) (p = 0.003) compared to NUD in 38(32%) while vacA s1bm1 was also associated with GU in 9(28%) (p = 0.001), DU in 12(37%) (p < 0.001) and GC 11(28%) (p < 0.001) compared to NUD in 13(11%), respectively. The diagnoses of GU in 21(66%), DU in 16(50%), GC in 20(50%) and NUD in 50(42%) were associated with moderately active chronic inflammation. CagA in 55(45%) (p = 0.037), vacA s1am1 in 51(51%) (P < 0.001), s1bm1 in 25(56%) (p = 0.002), s1am2 32(30%) (p < 0.001) and s1bm2 29(69%) (p = 0.004) were also associated with moderately active chronic inflammation.Conclusion: CagA was negative in majority of NUD patients with H. pylori infection. However, cagA was associated with peptic ulcer and GC. VacA allele's s1am1 and s1bm1 were associated with H. pylori associated diseases and inflammation. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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