A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival.
Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferati...
| Published in: | Wound Repair & Regeneration Vol. 18; no. 1; pp. 123 - 132 |
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| Main Authors: | , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Jan/Feb2010
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105286571&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105286571 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jan/Feb2010 vid: 18 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105286571 2010529215 10.1111/j.1524-475X.2009.00563.x 105286571 ppf: 123 ppct: 9 formats: fmt: @attributes: type: P tig: atl: A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival. aug: au: Tang L Cui T Wu JJ Liu-Mares W Huang N Li J affil: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida sug: subj: Fibroblasts Drug Effects Recombinant Proteins Therapeutic Use Wound Healing Drug Effects Coefficient alpha Cytokines Metabolism Data Analysis Software Funding Source Growth Substances Metabolism Human In Vitro Studies One-Way Analysis of Variance Paired T-Tests Tissue Culture Techniques Two-Way Analysis of Variance Unpaired T-Tests ab: Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferation. To explore the role of hLF in wound healing, we used recombinant human lactoferrin (holorhLF), derived from transgenic rice, to examine the effects of holo-rhLF on cell proliferation, migration, attachment, and survival in a human primary skin fibroblast culture system. This study revealed that holo-rhLF not only significantly stimulates fibroblast proliferation but also has synergistic effects with fibroblast growth factor-2 and antagonistic effects with transforming growth factor-ß1 on cell proliferation. Furthermore, using a chamber migration assay, our results demonstrate that holo-rhLF promotes fibroblast migration in a dosage-dependent manner. More importantly, holo-rhLF significantly increased cell viability and protected cells from death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant effect was observed on cell attachment. In conclusion, these findings reveal the multiple functions of holo-rhLF in human skin fibroblasts and indicate its potential application in wound therapy by enhancing cell proliferation and migration as well as protecting cells from apoptosis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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