In vitro and in vivo anti-Helicobacter pylori activity of Calophyllum brasiliense Camb.
AIMS OF THE STUDY: Calophyllum brasiliense (Camb.) is a medicinal tree that grows particularly in the hilly and forested regions of Brazil. Preparations from its stem bark are popular remedies for the treatment of chronic ulcers. Since earlier investigations on bark extracts evidenced gastroprotecti...
| Publicado en: | Journal of Ethnopharmacology Vol. 123; no. 3; pp. 452 - 459 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Elsevier B.V.
Jun2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105367271&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105367271 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Jun2009 vid: 123 iid: 3 pid: 1004 pub: Elsevier B.V. artinfo: ui: 105367271 2010325715 10.1016/j.jep.2009.03.030 NLM19501278 105367271 ppf: 452 ppct: 7 formats: tig: atl: In vitro and in vivo anti-Helicobacter pylori activity of Calophyllum brasiliense Camb. aug: au: Carmo Souza M Beserra AMS Martins DC Villa Real V Nunes Santos RA Rao VS Matos Silva R Oliveira Martins DT affil: Department of Basic Science in Health, Faculty of Medical Science, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa s/n, Coxipó, Cuiabá, MT CEP: 78060-900, Brazil. macsouza@hotmail.com sug: subj: Helicobacter Infections Prevention and Control Plant Bark Plant Extracts Administration and Dosage Plant Extracts Pharmacodynamics Plant Stems Plants, Medicinal Brazil Alternative Therapies Animal Studies Brazil Descriptive Statistics Fisher's Exact Test Funding Source In Vitro Studies In Vivo Studies One-Way Analysis of Variance Post Hoc Analysis Rats ab: AIMS OF THE STUDY: Calophyllum brasiliense (Camb.) is a medicinal tree that grows particularly in the hilly and forested regions of Brazil. Preparations from its stem bark are popular remedies for the treatment of chronic ulcers. Since earlier investigations on bark extracts evidenced gastroprotective and gastric acid inhibitory properties, this study evaluated the effects of hydroethanolic extract (HEECb) and the dichloromethanic fraction (DCMF), from Calophyllum brasiliense stem bark, against Helicobacter pylori, in vitro and in vivo. MATERIALS AND METHODS: The in vitro assays were performed using the disk diffusion and broth microdilution methods to determine the minimum inhibitory concentration (MIC) values. The test substances were evaluated in vivo taking into account the delay in the gastric ulcer healing in Wistar rats, infected with Helicobacter pylori. RESULTS: DCMF appeared the most active and potent in vitro against Helicobacter pylori growth with an MIC of 31 microg/mL. In the in vivo assays, rats ulcerated by acetic acid, and inoculated with Helicobacter pylori showed a marked delay in ulcer healing. Treatment with HEECb (50, 100 and 200 mg/kg) and DCMF (100 and 200 mg/kg) reduced the ulcerated area in a dose-dependent manner. While DCMF, at 200 mg/kg, increased the prostaglandin E2 (PGE2) level, both HEECb and DCMF decreased the number of urease-positive animals, as confirmed by the reduction of Helicobacter pylori presence in histopathological analysis. CONCLUSION: The results suggest that the antiulcer activity of Calophyllum brasiliense is due, in part, to its anti-Helicobacter pylori action, validating the popular use of this species. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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