Epithelial and stromal metabolite changes in the transition from cervical intraepithelial neoplasia to cervical cancer: an in vivo 1H magnetic resonance spectroscopic imaging study with ex vivo correlation.
To investigate epithelial and stromal metabolite changes in cervical intraepithelial neoplasia (CIN) and cervical cancer in vivo and correlate findings with MR spectroscopy of tissue samples. Forty-seven women (19 with CIN, 28 with cervical cancer) underwent endovaginal MR at 1.5 T with T2-W and loc...
| Publicado en: | European Radiology Vol. 19; no. 8; pp. 2041 - 2049 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2009
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105373548&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105373548 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Aug2009 vid: 19 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 105373548 42871646 NLM19259678 2010332413 10.1007/s00330-009-1363-0 NLM19259678 105373548 ppf: 2041 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Epithelial and stromal metabolite changes in the transition from cervical intraepithelial neoplasia to cervical cancer: an in vivo 1H magnetic resonance spectroscopic imaging study with ex vivo correlation. aug: au: De Silva SS Payne GS Morgan VA Ind TE Shepherd JH Barton DP Desouza NM De Silva, Sonali S Payne, Geoffrey S Morgan, Veronica A Ind, Thomas E J Shepherd, John H Barton, Desmond P J deSouza, Nandita M affil: Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK sug: subj: Cell Transformation, Neoplastic Metabolism Cervical Intraepithelial Neoplasia Metabolism Cervix Neoplasms Metabolism Connective Tissue Cells Metabolism Epithelial Cells Metabolism Magnetic Resonance Spectroscopy Methods Tumor Markers, Biological Analysis Adult Cell Transformation, Neoplastic Pathology Cervix Neoplasms Pathology Connective Tissue Cells Pathology Epithelial Cells Pathology Female Middle Age Protons Diagnostic Use Reproducibility of Results Sensitivity and Specificity Statistics Human Adult: 19-44 years Middle Aged: 45-64 years Female ab: To investigate epithelial and stromal metabolite changes in cervical intraepithelial neoplasia (CIN) and cervical cancer in vivo and correlate findings with MR spectroscopy of tissue samples. Forty-seven women (19 with CIN, 28 with cervical cancer) underwent endovaginal MR at 1.5 T with T2-W and localised 2D MR spectroscopic imaging (PRESS, TR = 1,500 ms, TE = 135 ms). tCho, 2 ppm and -CH(2) lipid peaks were measured in epithelial (>50% epithelium, no tumour), stromal (>50% stroma, no tumour) and tumour (>30% tumour) voxels. Unsuppressed water signal from the same voxel provided a concentration reference. (1)H HR-MAS MR spectra were acquired from tissue in 37 patients (11.74 T, pulse-acquire and cpmg sequences, with water pre-saturation). Analysable data from 17 CIN and 25 cancer patients showed significant increases in tCho (p = 0.03) and 2 ppm (p = 0.007) in tumour compared with epithelial voxels from CIN patients, but not with epithelial voxels from cancer patients. No significant differences were seen in stroma from cancer compared with CIN patients. Differences in -CH(2) lipids were not significant between groups. There was no significant correlation between in vivo and ex vivo tCho or -CH(2) lipids. Estimated in vivo concentrations of tCho and 2 ppm resonances increase in tumour and adjacent epithelium in progression from CIN to cervical cancer. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|