Gene transcript abundance profiles distinguish Kawasaki disease from adenovirus infection.
Background: Acute Kawasaki disease (KD) is difficult to distinguish from other illnesses that involve acute rash or fever, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed.Methods: We used DNA microar...
| Publicado en: | Journal of Infectious Diseases Vol. 200; no. 4; pp. 657 - 667 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
8/15/2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105407044&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105407044 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: 8/15/2009 vid: 200 iid: 4 pid: 622 pub: Oxford University Press / USA artinfo: ui: 105407044 NLM19583510 2010372036 10.1086/603538 NLM19583510 PMC2878183 105407044 ppf: 657 ppct: 10 formats: tig: atl: Gene transcript abundance profiles distinguish Kawasaki disease from adenovirus infection. aug: au: Popper SJ Watson VE Shimizu C Kanegaye JT Burns JC Relman DA Popper, Stephen J Watson, Virginia E Shimizu, Chisato Kanegaye, John T Burns, Jane C Relman, David A affil: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford,. CA 94305, USA sug: subj: DNA Virus Infections Diagnosis Genetic Techniques Mucocutaneous Lymph Node Syndrome Diagnosis B Lymphocytes Physiology Case Control Studies Cell Physiology Child DNA Virus Infections Genes Immunity Interferons Metabolism RNA Human Child: 6-12 years ab: Background: Acute Kawasaki disease (KD) is difficult to distinguish from other illnesses that involve acute rash or fever, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed.Methods: We used DNA microarrays to identify possible diagnostic features of KD. We compared gene expression patterns in the blood of 23 children with acute KD and 18 age-matched febrile children with 3 illnesses that resemble KD.Results: Genes associated with platelet and neutrophil activation were expressed at higher levels in patients with KD than in patients with acute adenovirus infections or systemic adverse drug reactions, but levels in patients with KD were not higher than those in patients with scarlet fever. Genes associated with B cell activation were also expressed at higher levels in patients with KD than in control subjects. A striking absence of interferon-stimulated gene expression in patients with KD was confirmed in an independent cohort of patients with KD. Using a set of 38 gene transcripts, we successfully predicted the diagnosis for 21 of 23 patients with KD and 7 of 8 patients with adenovirus infection.Conclusions: These findings provide insight into the molecular features that distinguish KD from other febrile illnesses and support the feasibility of developing novel diagnostic reagents for KD based on the host response. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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