Gamma tocopherol upregulates the expression of 15-S-HETE and induces growth arrest through a PPAR gamma-dependent mechanism in PC-3 human prostate cancer cells.
Chronic inflammation and dietary fat consumption correlates with an increase in prostate cancer. Our previous studies in the colon have demonstrated that gamma -tocopherol treatment could upregulate the expression of peroxisome proliferator-activated preceptors (PPAR) gamma , a nuclear receptor invo...
| Publicado en: | Nutrition & Cancer Vol. 61; no. 5; pp. 649 - 663 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Sep/Oct2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105431213&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105431213 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Sep/Oct2009 vid: 61 iid: 5 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 105431213 2010402474 10.1080/01635580902825654 NLM19838939 105431213 ppf: 649 ppct: 14 formats: fmt: @attributes: type: P tig: atl: Gamma tocopherol upregulates the expression of 15-S-HETE and induces growth arrest through a PPAR gamma-dependent mechanism in PC-3 human prostate cancer cells. aug: au: Campbell SE Musich PR Whaley SG Stimmel JB Leesnitzer LM Dessus-Babus S Duffourc M Stone W Newman RA Yang P Krishnan K affil: Department of Biochemistry, East Tennessee State University, P.O. Box 70851, Johnson City, TN 37614; campbese@etsu.edu sug: subj: Fatty Acids Therapeutic Use Prostatic Neoplasms Risk Factors Proteins Vitamin E Therapeutic Use Analysis of Variance Biological Assay Methods Blotting, Western Descriptive Statistics Enzyme-Linked Immunosorbent Assay Funding Source Neoplasms Physiopathology Nutrition Polymerase Chain Reaction Post Hoc Analysis Tissue Culture Techniques Methods Human ab: Chronic inflammation and dietary fat consumption correlates with an increase in prostate cancer. Our previous studies in the colon have demonstrated that gamma -tocopherol treatment could upregulate the expression of peroxisome proliferator-activated preceptors (PPAR) gamma , a nuclear receptor involved in fatty acid metabolism as well modulation of cell proliferation and differentiation. In this study, we explored the possibility that gamma -tocopherol could induce growth arrest in PC-3 prostate cancer cells through the regulation of fatty acid metabolism. Growth arrest (40%) and PPAR gamma mRNA and protein upregulation was achieved with gamma -tocopherol within 6 h. Gamma -Tocopherol-mediated growth arrest was demonstrated to be PPAR gamma dependent using the agonist GW9662 and a PPAR gamma dominant negative vector. Gamma -tocopherol was shown not to be a direct PPAR gamma ligand, but rather 15-S-HETE (an endogenous PPAR gamma ligand) was upregulated by gamma -tocopherol treatment. 15- Lipoxygenase-2, a tumor suppressor and the enzyme that converts arachidonic acid to 15-S-HETE, was upregulated at 3 h following gamma -tocopherol treatment. Expression of proteins downstream of the PPAR gamma pathway were examined. Cyclin D1, cyclin D3, bcl- 2, and NFkappa B proteins were found to be downregulated following gamma -tocopherol treatment. These data demonstrate that the growth arrest mediated by gamma -tocopherol follows a PPAR-gamma -dependent mechanism. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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