Computational challenges in miRNA target predictions: to be or not to be a true target?
All microRNA (miRNA) target--finder algorithms return lists of candidate target genes. How valid is that output in a biological setting? Transcriptome analysis has proven to be a useful approach to determine mRNA targets. Time course mRNA microarray experiments may reliably identify downregulated ge...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 9p - 10 |
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| Autores principales: | , , , , , |
| Formato: | review tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2009 Regular Issue
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105438742&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105438742 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2009 Regular Issue pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105438742 2010398058 10.1155/2009/803069 NLM19551154 105438742 ppf: 9p ppct: 1 formats: fmt: @attributes: type: P tig: atl: Computational challenges in miRNA target predictions: to be or not to be a true target? aug: au: Barbato C Arisi I Frizzo ME Brandi R Da Sacco L Masotti A affil: European Brain Research Institute-Fondazione EBRI-Rita Levi-Montalcini, Roma, Italy. sug: subj: Gene Expression Genes Genetic Techniques RNA Analysis of Variance Animals Biological Assay Funding Source Plants Proteins ab: All microRNA (miRNA) target--finder algorithms return lists of candidate target genes. How valid is that output in a biological setting? Transcriptome analysis has proven to be a useful approach to determine mRNA targets. Time course mRNA microarray experiments may reliably identify downregulated genes in response to overexpression of specific miRNA. The approach may miss some miRNA targets that are principally downregulated at the protein level. However, the high-throughput capacity of the assay makes it an effective tool to rapidly identify a large number of promising miRNA targets. Finally, loss and gain of function miRNA genetics have the clear potential of being critical in evaluating the biological relevance of thousands of target genes predicted by bioinformatic studies and to test the degree to which miRNA-mediated regulation of any 'validated' target functionally matters to the animal or plant. pubtype: Academic Journal doctype: review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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