Objective color scale for the SWYPE surface sampling technique using computerized image analysis tools.
Colorimetric SWYPE pads are useful tools for identifying unpolymerized aliphatic isocyanates on a variety of surfaces. This technique has been used in autobody shops to determine the presence of hexamethylene diisocyanate and other aliphatic isocyanates that are important constituents of many automo...
| Publicado en: | Journal of Occupational & Environmental Hygiene Vol. 6; no. 10; pp. 604 - 612 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Oct2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105443729&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105443729 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15459624 V1L jtl: Journal of Occupational & Environmental Hygiene issn: 15459624 maglogo: Y pubinfo: dt: Oct2009 vid: 6 iid: 10 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 105443729 105443729 2010403534 10.1080/15459620903117710 NLM19606376 105443729 ppf: 604 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Objective color scale for the SWYPE surface sampling technique using computerized image analysis tools. aug: au: Ceballos DM Yost MG Whittaker SG Camp J Dills R affil: Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA sug: subj: Environmental Monitoring Equipment and Supplies Environmental Monitoring Methods Occupational Exposure Evaluation Color Funding Source Industry Motor Vehicles Particulate Matter Reproducibility of Results Validity Human ab: Colorimetric SWYPE pads are useful tools for identifying unpolymerized aliphatic isocyanates on a variety of surfaces. This technique has been used in autobody shops to determine the presence of hexamethylene diisocyanate and other aliphatic isocyanates that are important constituents of many automotive coatings. SWYPEs have the advantage of being relatively inexpensive, rapid, and portable. The color change elicited by aliphatic isocyanates (from yellow to red) provides a visual indication of the extent of surface contamination. To quantify isocyanate contamination based on the colorimetric response, an objective color scale was developed for isocyanate loading. Sampled pads were digitized on a calibrated, portable flatbed scanner, and red-green-blue (RGB) histograms of SWYPE images were created. A calibration curve was created from a series of reference images derived from SWYPEs loaded with an isocyanate-containing product. The SWYPE RGB analysis allowed for quantification over a range of isocyanate loadings: from ~0.01 to 24.0 µg/cm², with reproducibility of >90%, accuracy >90%, and a surface extraction efficiency of >90%. RGB analysis exhibited a lower detection limit than visual scoring ( ~3 µg versus ~10 µg). The colorimetric response was cross validated with a high-performance liquid chromatography quantitative assay. When combined with RGB analysis, SWYPE colorimetric wipes represent a rapid and inexpensive method to assess objectively surface contamination with aliphatic isocyanates. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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