A controlled challenge study on di(2-ethylhexyl) phthalate (DEHP) in house dust and the immune response in human nasal mucosa of allergic subjects.
Background: Few studies have yet addressed the effects of di(2-ethylhexyl) phthalate (DEHP) in house dust on human nasal mucosa.Objectives: We investigated the effects of house dust containing DEHP on nasal mucosa of healthy and house dust mite (HDM) -- allergic subjects in a short-term exposure set...
| Published in: | Environmental Health Perspectives Vol. 116; no. 11; pp. 1487 - 1494 |
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| Main Authors: | , , , , , |
| Format: | clinical trial research tables/charts Journal Article |
| Published: |
National Institute of Environmental Health Sciences
Nov2008
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105570738&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105570738 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00916765 3B5 jtl: Environmental Health Perspectives issn: 00916765 maglogo: N pubinfo: dt: Nov2008 vid: 116 iid: 11 pid: 56539 pub: National Institute of Environmental Health Sciences place: Research Triangle Park, North Carolina artinfo: ui: 105570738 105570738 2010094834 10.1289/ehp.11474 NLM19057701 105570738 ppf: 1487 ppct: 7 formats: fmt: @attributes: type: P tig: atl: A controlled challenge study on di(2-ethylhexyl) phthalate (DEHP) in house dust and the immune response in human nasal mucosa of allergic subjects. aug: au: Deutschle T Reiter R Butte W Heinzow B Keck T Riechelmann H affil: Department of Otorhinolaryngology, University Hospital of Ulm, Frauensteige 14a, 89075 Ulm, Germany; tom.deutschle@uniklinik-ulm.de sug: subj: Hypersensitivity Risk Factors Nasal Mucosa Drug Effects Nasal Mucosa Immunology Polyvinyls Adverse Effects Adult Biochips Clinical Trials Data Analysis Software Eosinophils Female Funding Source Granulocyte Colony-Stimulating Factor Interleukins Male Mann-Whitney U Test Mites Random Assignment Wilcoxon Signed Rank Test Human Adult: 19-44 years Female Male ab: Background: Few studies have yet addressed the effects of di(2-ethylhexyl) phthalate (DEHP) in house dust on human nasal mucosa.Objectives: We investigated the effects of house dust containing DEHP on nasal mucosa of healthy and house dust mite (HDM) -- allergic subjects in a short-term exposure setting.Methods: We challenged 16 healthy and 16 HDM-allergic subjects for 3 hr with house dust at a concentration of 300 µg/m3 containing either low (0.41 mg/g) or high (2.09 mg/g) levels of DEHP. Exposure to filtered air served as control. After exposure, we measured proteins and performed a DNA microarray analysis.Results: Nasal exposure to house dust with low or high DEHP had no effect on symptom scores. Healthy subjects had almost no response to inhaled dust, but HDM-allergic subjects showed varied responses: DEHPlow house dust increased eosinophil cationic protein, granulocyte-colony--stimulating factor (G-CSF) , interleukin (IL) -5, and IL-6, whereas DEHPhigh house dust decreased G-CSF and IL-6. Furthermore, in healthy subjects, DEHP concentration resulted in 10 differentially expressed genes, whereas 16 genes were differentially expressed in HDM-allergic subjects, among them anti-Müllerian hormone, which was significantly up-regulated after exposure to DEHPhigh house dust compared with exposure to DEHPlow house dust, and fibroblast growth factor 9, IL-6, and transforming growth factor-beta1, which were down-regulated.Conclusions: Short-term exposure to house dust with high concentrations of DEHP has attenuating effects on human nasal immune response in HDM-allergic subjects, concerning both gene expression and cytokines. pubtype: Academic Journal doctype: clinical trial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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