Urinary levoglucosan as a biomarker of wood smoke exposure: observations in a mouse model and in children.

Background: Biomass smoke is an important source of particulate matter (PM), and much remains to be discovered with respect to the human health effects associated with this specific PM source. Exposure to biomass smoke can occur in one of two main categories: short-term exposures consist of periodic...

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Publicado en:Environmental Health Perspectives Vol. 117; no. 1; pp. 74 - 80
Autores principales: Migliaccio CT, Bergauff MA, Palmer CP, Jessop F, Noonan CW, Ward TJ
Formato: research tables/charts Journal Article
Publicado: National Institute of Environmental Health Sciences Jan2009
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jan2009
      vid: 117
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      pub: National Institute of Environmental Health Sciences
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        atl: Urinary levoglucosan as a biomarker of wood smoke exposure: observations in a mouse model and in children.
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          Migliaccio CT
          Bergauff MA
          Palmer CP
          Jessop F
          Noonan CW
          Ward TJ
        affil: Center for Environmental Health Sciences, Biomedical and Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, 285C Skaggs Building, University of Montana, Missoula, MT 59812 USA; christopher.migliaccio@umontana.edu
      sug:
        subj:
          Biological Markers Urine
          Environmental Exposure
          Glucans Urine
          Smoke Adverse Effects
          Analysis of Variance
          Animal Studies
          Child
          Convenience Sample
          Data Analysis Software
          Descriptive Statistics
          Funding Source
          In Vivo Studies
          Mice
          Montana
          Particulate Matter
          Pearson's Correlation Coefficient
          Pilot Studies
          T-Tests
          Urinalysis
          Human
          Child: 6-12 years
      ab: Background: Biomass smoke is an important source of particulate matter (PM), and much remains to be discovered with respect to the human health effects associated with this specific PM source. Exposure to biomass smoke can occur in one of two main categories: short-term exposures consist of periodic, seasonal exposures typified by communities near forest fires or intentional agricultural burning, and long-term exposures are chronic and typified by the use of biomass materials for cooking or heating. Levoglucosan (LG), a sugar anhydride released by combustion of cellulose-containing materials, is an attractive candidate as a biomarker of wood smoke exposure. Objectives: In the present study, Balb/c mice and children were assessed for LG in urine to determine its feasibility as a biomarker. Methods: We performed urinary detection of LG by gas chromatography/mass spectrometry after intranasal instillations of LG or concentrated PM (mice) or biomass exposure (mice or humans). Results: After instillation, we recovered most of the LG within the first 4 hr. Experiments using glucose instillation proved the specificity of our system, and instillation of concentrated PM from wood smoke, ambient air, and diesel exhaust supported a connection between wood smoke and LG. In addition, LG was detected in the urine of mice exposed to wood smoke. Finally, a pilot human study proved our ability to detect LG in urine of children. Conclusions: These results demonstrate that LG in the lungs is detectable in the urine of both mice and humans and that it is a good candidate as a biomarker of exposure to biomass smoke.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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