Gene expression profiling in Brodmann's area 46 from subjects with schizophrenia.
OBJECTIVE: To identify altered gene expression in the dorsolateral prefrontal cortex obtained after death from subjects with schizophrenia. METHOD: Restriction fragment differential display (RFDD) was used to measure levels of mRNA in Brodmann area (BA) 46 from schizophrenia and control subjects. Fi...
| Publicado en: | Australian & New Zealand Journal of Psychiatry Vol. 41; no. 4; pp. 308 - 321 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Sage Publications Inc.
Apr2007
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105654331&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105654331 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00048674 7ZU jtl: Australian & New Zealand Journal of Psychiatry issn: 00048674 maglogo: Y pubinfo: dt: Apr2007 vid: 41 iid: 4 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 105654331 105654331 2010032695 10.1080/00048670701213245 NLM17464717 105654331 ppf: 308 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Gene expression profiling in Brodmann's area 46 from subjects with schizophrenia. aug: au: Dean B Keriakous D Scarr E Thomas EA affil: Rebecca L. Cooper Research Laboratories, Mental Health Research Institute of Victoria, Locked Bag 11, Parkville, Vic. 3052, Australia. bdean_mhri@iprimus.com.au sug: subj: Frontal Lobe Metabolism Frontal Lobe Physiopathology Genetic Techniques Methods Proteins Metabolism Proteins Schizophrenia Metabolism Schizophrenia Physiopathology Schizophrenia Adult Animals Bipolar Disorder Metabolism Bipolar Disorder Physiopathology Bipolar Disorder DNA Female Male Nerve Tissue Proteins Metabolism Nerve Tissue Proteins Polymerase Chain Reaction Polymorphism, Genetic Rats RNA Signal Transduction Adult: 19-44 years Female Male ab: OBJECTIVE: To identify altered gene expression in the dorsolateral prefrontal cortex obtained after death from subjects with schizophrenia. METHOD: Restriction fragment differential display (RFDD) was used to measure levels of mRNA in Brodmann area (BA) 46 from schizophrenia and control subjects. Findings on specific mRNA identified with RFDD were further investigated using real-time polymerase chain reaction (real-time PCR), PCR and western blotting. RESULTS: Levels of mRNA for 63 of approximately 12,500 genes differed in BA 46 in schizophrenia. Subsequent real-time PCR has shown that mRNA for muscleblind protein 1 (MBNL1) and protocadherin 17 (PCDH17) are increased in BA 46 from subjects with schizophrenia of short, but not long, duration. Altered levels of mRNA for neither gene were present in BA 9 from subjects with schizophrenia or in either cortical area from subjects with bipolar 1 disorder. By contrast, both RFDD and real-time PCR failed to show altered expression of the schizophrenia candidate gene disrupted in schizophrenia 1 (DISC1) BA46 from any diagnostic cohort. CONCLUSION: The present study has identified genes that are differentially expressed in BA 46 in schizophrenia. Initial studies have shown that there is a need for a careful validation of genes shown to be affected in schizophrenia using high-throughput technologies. In addition the present study has shown that gene expression may vary considerably depending on the duration of schizophrenia. This raises the hypothesis that changing gene expression may be underlying the change in symptom profile that occurs with disease progression in some subjects with schizophrenia. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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