Alterations of the arginine metabolome in asthma.
Rationale: As the sole nitrogen donor in nitric oxide (NO) synthesis and key intermediate in the urea cycle, arginine and its metabolic pathways are integrally linked to cellular respiration, metabolism, and inflammation.Objectives: We hypothesized that arginine (Arg) bioavailability would be associ...
| Publicado en: | American Journal of Respiratory & Critical Care Medicine Vol. 178; no. 7; pp. 673 - 682 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
Oct2008
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105702165&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105702165 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1073449X 1FG jtl: American Journal of Respiratory & Critical Care Medicine issn: 1073449X maglogo: N pubinfo: dt: Oct2008 vid: 178 iid: 7 pid: 622 pub: Oxford University Press / USA artinfo: ui: 105702165 105702165 NLM18635886 2010097648 10.1164/rccm.200710-1542OC NLM18635886 PMC2556449 105702165 ppf: 673 ppct: 9 formats: tig: atl: Alterations of the arginine metabolome in asthma. aug: au: Lara A Khatri SB Wang Z Comhair SA Xu W Dweik RA Bodine M Levison BS Hammel J Bleecker E Busse W Calhoun WJ Castro M Chung KF Curran-Everett D Gaston B Israel E Jarjour N Moore W Peters SP sug: subj: Arginine Metabolism Asthma Metabolism Hydrolases Metabolism Nitric Oxide Metabolism Adult Arginine Blood Asthma Physiopathology Biological Availability Breath Tests Case Control Studies Female Hydrolases Blood Male Spirometry Human Adult: 19-44 years Female Male ab: Rationale: As the sole nitrogen donor in nitric oxide (NO) synthesis and key intermediate in the urea cycle, arginine and its metabolic pathways are integrally linked to cellular respiration, metabolism, and inflammation.Objectives: We hypothesized that arginine (Arg) bioavailability would be associated with airflow abnormalities and inflammation in subjects with asthma, and would be informative for asthma severity.Methods: Arg bioavailability was assessed in subjects with severe and nonsevere asthma and healthy control subjects by determination of plasma Arg relative to its metabolic products, ornithine and citrulline, and relative to methylarginine inhibitors of NO synthases, and by serum arginase activity. Inflammatory parameters, including fraction of exhaled NO (Fe(NO)), IgE, skin test positivity to allergens, bronchoalveolar lavage, and blood eosinophils, were also evaluated.Measurements and Main Results: Subjects with asthma had greater Arg bioavailability, but also increased Arg catabolism compared with healthy control subjects, as evidenced by higher levels of Fe(NO) and serum arginase activity. However, Arg bioavailability was positively associated with Fe(NO) only in healthy control subjects; Arg bioavailability was unrelated to Fe(NO) or other inflammatory parameters in severe or nonsevere asthma. Inflammatory parameters were related to airflow obstruction and reactivity in nonsevere asthma, but not in severe asthma. Conversely, Arg bioavailability was related to airflow obstruction in severe asthma, but not in nonsevere asthma. Modeling confirmed that measures of Arg bioavailabilty predict airflow obstruction only in severe asthma.Conclusions: Unlike Fe(NO), Arg bioavailability is not a surrogate measure of inflammation; however, Arg bioavailability is strongly associated with airflow abnormalities in severe asthma. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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