Expression of Ig-like transcript 4 inhibitory receptor in human non-small cell lung cancer.
Background: Human Ig-like transcript 4 (ILT-4) is a member of the inhibitory receptor family for immune function. Little is known about the expression levels of ILT-4 in tumor cells.Methods: We have studied the expression levels of ILT-4 both in vitro in cancer cell lines and in vivo in tumor tissue...
| Publicado en: | CHEST Vol. 134; no. 4; pp. 783 - 789 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American College of Chest Physicians
Oct2008
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105714138&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105714138 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00123692 1G4 jtl: CHEST issn: 00123692 maglogo: N pubinfo: dt: Oct2008 vid: 134 iid: 4 pid: 2556 pub: American College of Chest Physicians place: Glenview, Illinois artinfo: ui: 105714138 105714138 NLM18625675 2010070010 10.1378/chest.07-1100 NLM18625675 105714138 ppf: 783 ppct: 6 formats: tig: atl: Expression of Ig-like transcript 4 inhibitory receptor in human non-small cell lung cancer. aug: au: Sun Y Liu J Gao P Wang Y Liu C Sun, Yuping Liu, Jie Gao, Ping Wang, Yunshan Liu, Chuanyong affil: Jinan Central Hospital, Shandong University, Jinan, People's Republic of China sug: subj: Carcinoma, Non-Small-Cell Lung Metabolism Glycoproteins Metabolism Lung Neoplasms Metabolism Receptors, Cell Surface Metabolism Aged Antigens, Surface Metabolism Carcinoma, Non-Small-Cell Lung Immunology Carcinoma, Non-Small-Cell Lung Pathology Cells Female Glycoproteins Lung Neoplasms Immunology Lung Neoplasms Pathology Lymphocytes Physiology Male Middle Age Neoplasm Staging Prospective Studies Receptors, Cell Surface RNA Metabolism Human Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Background: Human Ig-like transcript 4 (ILT-4) is a member of the inhibitory receptor family for immune function. Little is known about the expression levels of ILT-4 in tumor cells.Methods: We have studied the expression levels of ILT-4 both in vitro in cancer cell lines and in vivo in tumor tissues from 70 patients with non-small cell lung cancer (NSCLC) by reverse transcriptase-polymerase chain reaction, fluorescence-activated cell sorting, and immunohistochemical analysis.Results: Three cancer cell lines (H1299, A549, and U1810) express ILT-4 messenger RNA, and only two cell lines (H1299 and A549) express ILT-4 protein on the cell surface. Approximately 37.1% of 70 tumor tissue samples express ILT-4, which is localized in the cell membrane and cytoplasm. In addition, tumor cells and stromal and plasma cells also express ILT-4. The number of infiltrating lymphoid cells in the tumor tissues that express B7-H3 was much lower than those that did not, but there is no significant correlation between ILT-4 expression and disease progression including nodal metastasis.Conclusions: These findings suggest that ILT-4 is frequently expressed in both tumor and stromal cells of NSCLC, and it might play an important role in regulation of the host immune system. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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