Intense noise-induced apoptosis in hair cells of Guinea pig cochleae.
Cells can die by two distinct pathways: apoptosis and necrosis. To explore whether intense noise can induce hair cell (HC) death via the apoptotic pathway, we systematically examined morphological changes in guinea pig cochlear HC nuclei stained with Hoechst 33342, a fluorescent dye specifically lab...
| Publicado en: | Acta Oto-Laryngologica Vol. 120; no. 1; pp. 19 - 25 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Taylor & Francis Ltd
1/1/2000
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105716436&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105716436 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: 1/1/2000 vid: 120 iid: 1 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 105716436 105716436 2009394391 NLM10779180 105716436 ppf: 19 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Intense noise-induced apoptosis in hair cells of Guinea pig cochleae. aug: au: Hu BH Guo W Wang PY Henderson D Jiang SC sug: subj: Apoptosis Physiology Cochlea Pathology Noise Adverse Effects Animal Studies Auditory Threshold Evoked Potentials, Auditory, Brainstem Physiology Guinea Pigs Hearing Loss, Noise-Induced Diagnosis Hearing Loss, Noise-Induced Etiology Models, Biological Random Assignment Severity of Illness Indices ab: Cells can die by two distinct pathways: apoptosis and necrosis. To explore whether intense noise can induce hair cell (HC) death via the apoptotic pathway, we systematically examined morphological changes in guinea pig cochlear HC nuclei stained with Hoechst 33342, a fluorescent dye specifically labelling the nuclear DNA. A narrow band noise centred at 4 kHz with levels at 110 dB, 115 dB or 120 dB (SPL) was applied for 4 h and the exposed cochleae were collected at various intervals (3 h, 3 or 14 days) after the noise exposure. Auditory function was monitored by measuring thresholds of auditory brain stem responses. In the noise-damaged cochleae, there were two major types of nuclear changes, nuclear condensation appeared as karyorrhexis or karyopyknosis and nuclear swelling. Karyorrhexis and karyopyknosis predominately appeared in the severely damaged cochlear region in the animals exposed to 120 dB noise and examined 3 h after the noise exposure. In contrast, swelling of nuclei occurred in all of the noise-exposed cochleae, and was the feature change in the animals exposed to 110 and 115 dB noise. This pathological change persisted at least for 14 days after the noise exposure. The typical changes of karyorrhexis and karyopyknosis noted in the animals exposed to 120 dB noise were morphologically similar to those nuclear changes described in previous studies for apoptosis, suggesting that the apoptotic process may be involved in intense noise-induced HC death. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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