A better method for confirming Helicobacter pylori infection in Mongolian gerbils.
Background: Our aim was to evaluate the accuracy of the stool antigen test and the optimal time point for detecting Helicobacter pylori infection in a Mongolian gerbil model.Methods: We inoculated 8-week-old Mongolian gerbils with H. pylori (Vac A (+)/CagA(+)). The gerbil-infected model was develope...
| Publicado en: | Journal of Gastroenterology Vol. 43; no. 1; pp. 32 - 38 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
2008
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105733699&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105733699 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09441174 EJ7 jtl: Journal of Gastroenterology issn: 09441174 maglogo: N pubinfo: dt: 2008 vid: 43 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 105733699 NLM18297433 2009806197 10.1007/s00535-007-2121-1 NLM18297433 105733699 ppf: 32 ppct: 6 formats: tig: atl: A better method for confirming Helicobacter pylori infection in Mongolian gerbils. aug: au: Kuo C Hu H Tsai P Yang S Chang L Wang J Chen A Jan C Wang W Wu D Kuo, Chao-Hung Hu, Huang-Ming Tsai, Pei-Yun Yang, Sheau-Fang Chang, Lin-Li Wang, Jaw-Yuan Chen, Angela Jan, Chang-Ming Wang, Wen-Ming Wu, Deng-Chyang affil: Division of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan sug: subj: Helicobacter Infections Helicobacter Pylori Animals Chi Square Test Funding Source Inflammation Intestine, Small Anatomy and Histology Polymerase Chain Reaction Stomach Neoplasms Stomach Anatomy and Histology Taiwan Animal Studies ab: Background: Our aim was to evaluate the accuracy of the stool antigen test and the optimal time point for detecting Helicobacter pylori infection in a Mongolian gerbil model.Methods: We inoculated 8-week-old Mongolian gerbils with H. pylori (Vac A (+)/CagA(+)). The gerbil-infected model was developed as follows: H. pylori was put into broth (about 10(9) CFU/ml), and 50 gerbils were then fed with 1 ml intragastrically twice within a 3-day interval. Another ten gerbils were fed broth only. Twenty-six weeks after the inoculation, the gerbils were killed. The gastric mucosa was sampled for a series of examinations including culture, histology, rapid urease test, and polymerase chain reaction. Stool samples for a stool antigen test, H. pylori-specific stool antigen assay (HpSA), were collected during weeks 4, 6, 8, 12, and 26 after inoculation. Of the 50 gerbils inoculated with H. pylori, the inoculation was successful in 88%. Severe active gastritis, ulceration, and intestinal metaplasia were obvious.Results: The HpSA test results were sensitivity, 88.6%; specificity, 100%; positive predictive value (PPV), 100%; negative predictive value (NPV), 54.5%, and accuracy, 90%. The HpSA test began to be more sensitive and accurate (P < 0.05) beginning during week 6 after inoculation. We also found that H. pylori could be detected earlier and more easily in the group with high H. pylori density.Conclusions: HpSA seems to be suitable for confirming colonization of gerbils with H. pylori. The optimal testing time point is around 6 weeks after inoculation. This test is a good choice for long-term observation of H. pylori infection in Mongolian gerbils. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|