I(Ks) block by HMR 1556 lowers ventricular defibrillation threshold and reverses the repolarization shortening by isoproterenol without rate-dependence in rabbits.

INTRODUCTION: The slow delayed rectifier K+ current (I(Ks)) contributes little to ventricular repolarization at rest. It is unclear whether I(Ks) plays a role during ventricular fibrillation (VF) or ventricular repolarization at rapid rates during beta-adrenergic stimulation. METHODS AND RESULTS: In...

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Publicado en:Journal of Cardiovascular Electrophysiology Vol. 18; no. 7; pp. 750 - 757
Autores principales: So PP, Backx PH, Hu XD, Dorian P
Formato: Journal Article
Publicado: Wiley-Blackwell Jul2007
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jul2007
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: I(Ks) block by HMR 1556 lowers ventricular defibrillation threshold and reverses the repolarization shortening by isoproterenol without rate-dependence in rabbits.
      aug:
        au:
          So PP
          Backx PH
          Hu XD
          Dorian P
        affil: Division of Cardiology, St. Michael's Hospital, Toronto, Ontario, Canada.
      sug:
        subj:
          Action Potentials Drug Effects
          Benzopyrans Pharmacodynamics
          Cardioversion Methods
          Isoproterenol Pharmacodynamics
          Membrane Proteins Antagonists and Inhibitors
          Sulfonamides Pharmacodynamics
          Action Potentials Physiology
          Animals
          Benzopyrans Therapeutic Use
          Male
          Membrane Proteins Physiology
          Rabbits
          Sulfonamides Therapeutic Use
          Ventricular Fibrillation Drug Therapy
          Ventricular Fibrillation Physiopathology
          Male
      ab: INTRODUCTION: The slow delayed rectifier K+ current (I(Ks)) contributes little to ventricular repolarization at rest. It is unclear whether I(Ks) plays a role during ventricular fibrillation (VF) or ventricular repolarization at rapid rates during beta-adrenergic stimulation. METHODS AND RESULTS: In an in vivo rabbit model, we evaluated the effects of HMR 1556 (1 mg Kg(-1) + 1 mg kg(-1) hr(-1) i.v.), a selective I(Ks) blocker, on monophasic action potential duration at 90% repolarization (MAPD90), ventricular effective refractory period (VERP), and defibrillation threshold (DFT). In perfused rabbit hearts, the effects of HMR 1556 (10 and 100 nM) in the presence of isoproterenol (5 nM) on MAPD90 and VERP were studied at cycle lengths (CLs) 200-500 msec. In vivo, HMR 1556 prolonged MAPD90 by 6 +/- 1 msec at CL 200 msec (P < 0.01, n = 6), lowered DFT from 558 +/- 46 V to 417 +/- 31 V (P < 0.01), and decreased the coefficient of variation in the VF inter-beat deflection intervals from 8.9 +/- 0.6% to 6.5 +/- 0.4% (P < 0.05) compared with control. In perfused rabbit hearts, isoproterenol shortened MAPD90 by 5 +/- 1 msec at CL 200 msec and 11 +/- 4 msec at CL 500 msec (P < 0.05, n = 7). This shortening was reversed by HMR 1556 (P < 0.05), and both effects were rate-independent. CONCLUSION: I(Ks) block increases VF temporal organization and lowers DFT, and I(Ks) that is activated following beta-adrenergic stimulation contributes to ventricular repolarization without rate dependence.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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