Inhalation exposure study of titanium dioxide nanoparticles with a primary particle size of 2 to 5 nm.

Background: Nanotechnology offers great promise in many industrial applications. However, little is known about the health effects of manufactured nanoparticles, the building blocks of nanomaterials.Objectives: Titanium dioxide (TiO[2]) nanoparticles with a primary size of 2-5 nm have not been studi...

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Publicado en:Environmental Health Perspectives Vol. 115; no. 3; pp. 397 - 403
Autores principales: Grassian VH, O'Shaughnessy PT, Adamcakova-Dodd A, Pettibone JM, Thorne PS
Formato: research Journal Article
Publicado: National Institute of Environmental Health Sciences Mar2007
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Mar2007
      vid: 115
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      pub: National Institute of Environmental Health Sciences
      place: Research Triangle Park, North Carolina
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        atl: Inhalation exposure study of titanium dioxide nanoparticles with a primary particle size of 2 to 5 nm.
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          Grassian VH
          O'Shaughnessy PT
          Adamcakova-Dodd A
          Pettibone JM
          Thorne PS
        affil: Department of Chemistry, University of Iowa, Iowa City, IA 52242; vicki-grassian@uiowa.edu
      sug:
        subj:
          Inhalation Exposure
          Lung Drug Effects
          Nanotechnology
          Titanium
          Animal Studies
          Animals
          Body Fluids
          Body Fluids Immunology
          Cytokines Immunology
          Environmental Exposure
          Leukocyte Count
          Lung Immunology
          Macrophages Drug Effects
          Macrophages Immunology
          Male
          Mice
          Particle Size
          Titanium Administration and Dosage
          Male
      ab: Background: Nanotechnology offers great promise in many industrial applications. However, little is known about the health effects of manufactured nanoparticles, the building blocks of nanomaterials.Objectives: Titanium dioxide (TiO[2]) nanoparticles with a primary size of 2-5 nm have not been studied previously in inhalation exposure models and represent some of the smallest manufactured nanoparticles. The purpose of this study was to assess the toxicity of these nanoparticles using a murine model of lung inflammation and injury.Materials and Methods: The properties of TiO[2] nanoparticles as well as the characteristics of aerosols of these particles were evaluated. Mice were exposed to TiO[2] nanoparticles in a whole-body exposure chamber acutely (4 hr) or subacutely (4 hr/day for 10 days). Toxicity in exposed mice was assessed by enumeration of total and differential cells, determination of total protein, lactate dehydrogenase (LDH) activity and inflammatory cytokines in bronchoalveolar lavage (BAL) fluid. Lungs were also evaluated for histopathologic changesResults: Mice exposed acutely to 0.77 or 7.22 mg/m[3] nanoparticles demonstrated minimal lung toxicity or inflammation. Mice exposed subacutely (8.88 mg/m[3]) and necropsied immediately and at week 1 or 2 postexposure had higher counts of total cells and alveolar macrophages in the BAL fluid compared with sentinels. However, mice recovered by week 3 postexposure. Other indicators were negative.Conclusions: Mice subacutely exposed to 2-5 nm TiO[2] nanoparticles showed a significant but moderate inflammatory response among animals at week 0, 1, or 2 after exposure that resolved by week 3 postexposure.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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