In vivo imaging of dendritic spines during electrographic seizures.
Epilepsy is associated with significant neurological morbidity, including learning disabilities, motor deficits, and behavioral problems. Although the causes of neurological dysfunction in epilepsy are multifactorial, accumulating evidence indicates that seizures in themselves may directly cause bra...
| Publicado en: | Annals of Neurology Vol. 58; no. 6; pp. 888 - 899 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
Dec2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106021889&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106021889 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03645134 1WM jtl: Annals of Neurology issn: 03645134 maglogo: Y pubinfo: dt: Dec2005 vid: 58 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 106021889 2009580148 NLM16240365 106021889 ppf: 888 ppct: 11 formats: tig: atl: In vivo imaging of dendritic spines during electrographic seizures. aug: au: Rensing N Ouyang Y Yang XF Yamada KA Rothman SM Wong M affil: Department of Neurology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA. sug: subj: Neurons Pathology Neurons Physiology Epilepsy Pathology Epilepsy Physiopathology Aminopyridines Anesthesia Animals Convulsants Electroencephalography Epilepsy Chemically Induced Proteins Lasers Mice Cerebral Cortex Pathology Cerebral Cortex Physiopathology Wakefulness ab: Epilepsy is associated with significant neurological morbidity, including learning disabilities, motor deficits, and behavioral problems. Although the causes of neurological dysfunction in epilepsy are multifactorial, accumulating evidence indicates that seizures in themselves may directly cause brain injury. Although it is clear that seizures can result in neuronal death, it is likely that under some circumstances seizures can induce more subtle functional or structural alterations in neurons. We induced focal neocortical seizures with 4-aminopyridine in transgenic mice expressing green fluorescent protein in cortical neurons and sequentially imaged individual dendrites in living animals with two-photon laser-scanning microscopy to determine whether these seizures caused acute alterations in dendritic spine morphology. No dendritic alterations were observed in anesthetized animals during electrographic seizures over a 3-hour period. Similarly, in unanesthetized mice, low-stage, clinical electrographic seizures had minimal effect on dendritic spines. More severe, high-stage seizures in unanesthetized mice were associated with a moderate loss of spines and dendritic swelling, but this effect may have been contingent on a synergistic action of phototoxicity from the imaging method itself. Overall, our results suggest that most neocortical seizures have minimal acute effects on dendrites over several hours, but may predispose to dendritic injury under extreme conditions. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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