Cellular proliferative fraction measured with topoisomerase iia predicts Malignancy in endocrine pancreatic tumors.

Context.-Endocrine pancreatic tumors (EPTs) are rare lesions with varying biological behavior. Establishing malignancy is a challenge for clinicians and pathologists. Objective.-To establish the role of proliferative, apoptotic, angiogenic, and hormonal markers as predictors of malignancy in EPTs. D...

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Publicado en:Archives of Pathology & Laboratory Medicine Vol. 128; no. 4; pp. 426 - 430
Autores principales: Diaz-Rubio JL, Duarte-Rojo A, Saqui-Salces M, Gamboa-Dominguez A, Robles-Diaz G
Formato: research Journal Article
Publicado: College of American Pathologists Apr2004
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Apr2004
      vid: 128
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      pub: College of American Pathologists
      place: Northfield, Illinois
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        atl: Cellular proliferative fraction measured with topoisomerase iia predicts Malignancy in endocrine pancreatic tumors.
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          Diaz-Rubio JL
          Duarte-Rojo A
          Saqui-Salces M
          Gamboa-Dominguez A
          Robles-Diaz G
      sug:
        subj:
          Enzymes Analysis
          Gastrinoma Pathology
          Insulinoma Pathology
          Pancreatic Neoplasms Pathology
          Proteins Analysis
          Tumor Markers, Biological Analysis
          Adolescence
          Adult
          Aged
          Antigens, Tumor
          Apoptosis
          Cell Physiology
          Endothelial Growth Factors Analysis
          Female
          Gastrinoma Blood Supply
          Gastrinoma
          Genetic Techniques
          Insulinoma Blood Supply
          Insulinoma
          Male
          Middle Age
          Neoplasm Invasiveness
          Neovascularization, Pathologic Pathology
          Pancreatic Neoplasms
          Prognosis
          Proteins
          ROC Curve
          Sensitivity and Specificity
          Human
          Adolescent: 13-18 years
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Context.-Endocrine pancreatic tumors (EPTs) are rare lesions with varying biological behavior. Establishing malignancy is a challenge for clinicians and pathologists. Objective.-To establish the role of proliferative, apoptotic, angiogenic, and hormonal markers as predictors of malignancy in EPTs. Design.-Paraffin-embedded EPT samples were studied for prognostic markers. Patients.-Twenty-one consecutive patients with a diagnosis of EPT. Main Outcome Measures.-The proliferative fraction (topoisomerase Ii[alpha]), microvascular density (CD34), vascular endothelial growth factor expression, and estrogen receptor-beta (ER[beta]) expression were studied by immunohistochemistry on all EPTs. Apoptosis was also assessed with terminal deoxynucleotidyl transferase nick-end labeling. Results.-We identified 13 benign and 8 malignant tumors. Topoisomerase II[alpha] was significantly increased in malignant tumors ( P = .001), while there were no differences in apoptosis, microvascular density, or vascular endothelial growth factor expression in association with malignancy. No correlation could be identified between microvascular density and vascular endothelial growth factor expression, and ER[beta] was not detected. A receiver operating characteristic curve for topoisomerase II[alpha] disclosed that above a labeling index of 13, the test had 88% sensitivity and 100% specificity for predicting malignancy. Conclusion.-Cellular proliferation measured with topoisomerase II[alpha] is a simple prognostic marker for malignancy in EPTs, unlike apoptosis, angiogenesis, or the presence of ER[beta], which were not associated with malignant behavior. These findings designate a defined field for future research on endocrine pancreatic carcinogenesis and a possible target for chemotherapeutic agents.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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