Chemopreventive Effects of Cuminum cyminum in Chemically Induced Forestomach and Uterine Cervix Tumors in Murine Model Systems.

Lately a strong correlation has been established between diet and cancer. For ages, cumin has been a part of the diet. It is a popular spice regularly used as a flavoring agent in a number of ethnic cuisines. In the present study cancer chemopreventive potentials of different doses of a cumin seed-m...

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Publicado en:Nutrition & Cancer Vol. 47; no. 2; pp. 171 - 181
Autores principales: Dhanalakshmi S, Méndiz E, Rao AR, Kale RK
Formato: research Journal Article
Publicado: Taylor & Francis Ltd 2003
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2003
      vid: 47
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        atl: Chemopreventive Effects of Cuminum cyminum in Chemically Induced Forestomach and Uterine Cervix Tumors in Murine Model Systems.
      aug:
        au:
          Dhanalakshmi S
          Méndiz E
          Rao AR
          Kale RK
      sug:
        subj:
          Antineoplastic Agents Therapeutic Use
          Cervix Neoplasms Prevention and Control
          Medicine, Herbal
          Plant Extracts Therapeutic Use
          Plants
          Stomach Neoplasms Prevention and Control
          Animal Studies
          Cervix Neoplasms Chemically Induced
          Diet
          Dose-Response Relationship, Drug
          Female
          Hemeproteins Metabolism
          Lipid Peroxidation Drug Effects
          Liver
          Liver Drug Effects
          Mice
          Oxidative Stress Drug Effects
          Oxidoreductases Metabolism
          Random Assignment
          Stomach Neoplasms Chemically Induced
          Superoxide Dismutase Metabolism
          Transferases Metabolism
          Female
      ab: Lately a strong correlation has been established between diet and cancer. For ages, cumin has been a part of the diet. It is a popular spice regularly used as a flavoring agent in a number of ethnic cuisines. In the present study cancer chemopreventive potentials of different doses of a cumin seed-mixed diet were evaluated against benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis and 3-methylcholanthrene (MCA)-induced uterine cervix tumorigenesis. Results showed a significant inhibition of stomach tumor burden (tumors per mouse) by cumin. Tumor burden was 7.33 ± 2.10 in the B(a)P-treated control whereas it reduced to 3.10 ± 0.57 (P < 0.001) by a 2.5% dose and 3.11 ± 0.60 (P <0.001) by a 5% dose of cumin seeds. Cervical carcinoma incidence, compared with the MCA-treated control group (66.67%), reduced to 27.27% (P < 0.05) by a diet of 5% cumin seeds and to 12.50% (P < 0.05) by a diet of 7.5% cumin seeds. The effect of 2.5 and 5% cumin seed-mixed diets was also examined on carcinogen/xenobiotic metabolizing phase I and phase II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase (LDH), and lipid peroxidation in the liver of Swiss albino mice. Levels of cytochrome P-450 (cyt P-450) and cytochrome b5 (cyt b5) were significantly augmented (P < 0.05) by the 2.5% dose of cumin seed diet. The levels of cyt P-450 reductase and cyt b5 reductase were increased (significance level being from P < 0.05 to P < 0.01) by both doses of cumin. Among the phase II enzymes, glutathione S-transferase specific activity increased (P < 0.005) by the 5% dose, whereas that of DT-diaphorase increased significantly (P < 0.05) by both doses used (2.5 and 5%). In the antioxidant system, significant elevation of the specific activities of super-oxide dismutase (P < 0.01) and catalase (P < 0.05) was observed with the 5% dose of cumin. The activities of glutathione peroxidase and glutathione reductase remained unaltered by both doses of cumin. The level of reduced elutathione measured as nonprotein sulfhydryl content was elevated (significance level being from P < 0.05 to P < 0.01) by both doses of cumin. Lipid peroxidation measured as formation of MDA production showed significant inhibition (P < 0.05 to P < 0.01) by both doses of cumin. LDH activity remained unaltered by both doses of cumin. The results strongly suggest the cancer chemopreventive potentials of cumin seed and could be attributed to its ability to modulate carcinogen metabolism.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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