Transmural distribution of connexins in rodent hearts.
Transmural Distribution of Connexins. Introduction: Electrophysiologic heterogeneity across the ventricular wall is a result of differential transmural expression of various ion channel proteins that underlie the different action potential waveforms observed in epicardial, midmyocardial, and endocar...
| Publicado en: | Journal of Cardiovascular Electrophysiology Vol. 15; no. 6; pp. 710 - 716 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
Jun2004
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106081908&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106081908 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10453873 GSB jtl: Journal of Cardiovascular Electrophysiology issn: 10453873 maglogo: Y pubinfo: dt: Jun2004 vid: 15 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 106081908 2009396044 NLM15175068 106081908 ppf: 710 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Transmural distribution of connexins in rodent hearts. aug: au: Yamada KA Kanter EM Green KG Saffitz JE sug: subj: Membrane Proteins Metabolism Myocardium Metabolism Animals Endocardium Metabolism Fluorescent Antibody Technique Heart Function Tests Heart Ventricle Metabolism Immunoblotting Mice Models, Biological Rats Rodents ab: Transmural Distribution of Connexins. Introduction: Electrophysiologic heterogeneity across the ventricular wall is a result of differential transmural expression of various ion channel proteins that underlie the different action potential waveforms observed in epicardial, midmyocardial, and endocardial regions. Cardiac connexins mediate cell-to-cell communication, are critical for normal impulse propagation, and play a role in electrophysiologic remodeling in disease states. However, little is known about the transmural distribution of cardiac gap junction proteins. Methods and Results: Connexin expression in epicardium, midmyocardium, and endocardium was assessed immunohistochemically in mouse and rat hearts. The total connexin protein content within different ventricular regions was measured by immunoblotting. Connexin43 is twice as abundant in midmyocardium and endocardium compared with epicardium in the mouse but not in the rat. Connexin45 is expressed equally across the left ventricular wall. Conclusion: Epicardial myocytes express significantly less Cx43 and therefore may be less well coupled than midmyocardial and endocardial myocytes. A transmural gradient of connexin43 expression across the left ventricular free wall likely results in differences in the stoichiometry of connexins expressed in different regions of the heart. (J Cardiovasc Electrophysiol, Vol. 15, pp. 1-6, June 2004) pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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