Chemotherapy-induced apoptosis and Bcl-2 levels correlate with breast cancer response to chemotherapy.
Purpose: The relevance of apoptosis to breast cancer response to chemotherapy is unclear. We investigated whether changes in tumor cell apoptosis and Bcl-2 expression immediately after chemotherapy correlated with response to breast cancer treatment.Patients and Methods: Serial core biopsies of 25 b...
| Publicado en: | Cancer Journal Vol. 9; no. 1; pp. 33 - 34 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Jan/Feb2003
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106099433&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106099433 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15289117 4U5 jtl: Cancer Journal issn: 15289117 maglogo: N pubinfo: dt: Jan/Feb2003 vid: 9 iid: 1 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 106099433 NLM12602766 2009455913 10.1097/00130404-200301000-00007 NLM12602766 106099433 ppf: 33 ppct: 1 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Chemotherapy-induced apoptosis and Bcl-2 levels correlate with breast cancer response to chemotherapy. aug: au: Buchholz TA Davis DW Mcconkey DJ Symmans WF Valero V Jhingran A Tucker SL Pusztai L Cristofanilli M Esteva FJ Hortobagyi GN Sahin AA Buchholz, Thomas A Davis, Darren W McConkey, David J Symmans, W Fraser Valero, Vicente Jhingran, Anuja Tucker, Susan L Pusztai, Lajos affil: Department of Radiation Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA sug: subj: Antineoplastic Agents, Combined Therapeutic Use Apoptosis Drug Effects Breast Neoplasms Drug Therapy Doxorubicin Therapeutic Use Hydrocarbons Paclitaxel Analogs and Derivatives Paclitaxel Therapeutic Use Proteins Metabolism Adult Aged Biopsy Methods Breast Neoplasms Pathology Breast Neoplasms Surgery Female Genetic Techniques Middle Age Neoplastic Processes Drug Therapy Neoplastic Processes Pathology Neoplastic Processes Surgery Predictive Value of Tests Prospective Studies Time Factors Tumor Markers, Biological Metabolism Human Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female ab: Purpose: The relevance of apoptosis to breast cancer response to chemotherapy is unclear. We investigated whether changes in tumor cell apoptosis and Bcl-2 expression immediately after chemotherapy correlated with response to breast cancer treatment.Patients and Methods: Serial core biopsies of 25 breast cancer primary tumors were performed at either two or three time points: before treatment (N = 24) and approximately 24 hours (N = 22) and/or 48 hours (N = 19) after the initiation of the first cycle of chemotherapy. Apoptosis levels were quantified by use of a fluorescent terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) stain, and Bcl-2 and Bax were measured by semiquantitative immunohistochemical assays. All calculated P values were two sided.Results: The apoptosis level at 48 hours was significantly higher in the tumors with pathological complete response or < 1 cm of residual disease (median, 22%; range, 6%-51%) than in the tumors with > 1 cm residual disease (median, 7%; range, 1%-36%); Mann-Whitney test. This difference was also present in the subgroup of 16 tumors treated with docetaxel/doxorubicin chemotherapy (25% vs 4%, respectively). A decrease in Bcl2 expression after chemotherapy relative to the expression from the pretreatment sample also correlated with disease response. Specifically, three of the nine tumors with a decrease in Bcl-2 had a pathological complete response, compared with 0 of the 15 tumors with stable levels of Bcl-2 (Fisher's exact test). There was no relationship between serial measurements of Bax and response.Discussion: These data suggest that apoptosis may play an important role in determining breast cancer response to chemotherapy and that the level of treatment-induced apoptosis may have some value as a predictive marker. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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