Correlation of in vitro cytokine responses with the chemical composition of soil-derived particulate matter.
We treated human lung epithelial cells, type BEAS-2B, with 10-80 microg/cm2 of dust from soils and road surfaces in the western United States that contained particulate matter (PM) < 2.5 microm aerodynamic diameter. Cell viability and cytokine secretion responses were measured at 24 hr. Each dust sa...
| Publicado en: | Environmental Health Perspectives Vol. 114; no. 3; pp. 341 - 350 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
National Institute of Environmental Health Sciences
Mar2006
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106141020&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106141020 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00916765 3B5 jtl: Environmental Health Perspectives issn: 00916765 maglogo: N pubinfo: dt: Mar2006 vid: 114 iid: 3 pid: 56539 pub: National Institute of Environmental Health Sciences place: Research Triangle Park, North Carolina artinfo: ui: 106141020 106141020 2009260222 10.1289/ehp.8360 NLM16507455 106141020 ppf: 341 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Correlation of in vitro cytokine responses with the chemical composition of soil-derived particulate matter. aug: au: Veranth JM Moss TA Chow JC Labban R Nichols WK Walton JC Watson JG Yost GS affil: Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84112-5820, USA. John.Veranth@utah.edu sug: subj: Air Pollutants Dust Analysis Environmental Pollutants Interleukins Air Pollutants Analysis Carbon Analysis Cell Physiology Drug Effects Cells Data Analysis Software Environmental Monitoring Environmental Pollutants Analysis Epithelial Cells Drug Effects Epithelial Cells Metabolism Funding Source Manganese Analysis Human ab: We treated human lung epithelial cells, type BEAS-2B, with 10-80 microg/cm2 of dust from soils and road surfaces in the western United States that contained particulate matter (PM) < 2.5 microm aerodynamic diameter. Cell viability and cytokine secretion responses were measured at 24 hr. Each dust sample is a complex mixture containing particles from different minerals mixed with biogenic and anthropogenic materials. We determined the particle chemical composition using methods based on the U.S. Environmental Protection Agency Speciation Trends Network (STN) and the National Park Service Interagency Monitoring of Protected Visual Environments (IMPROVE) network. The functionally defined carbon fractions reported by the ambient monitoring networks have not been widely used for toxicology studies. The soil-derived PM2.5 from different sites showed a wide range of potency for inducing the release of the proinflammatory cytokines interleukin-6 (IL-6) and IL-8 in vitro. Univariate regression and multivariate redundancy analysis were used to test for correlation of viability and cytokine release with the concentrations of 40 elements, 7 ions, and 8 carbon fractions. The particles showed positive correlation between IL-6 release and the elemental and pyrolyzable carbon fractions, and the strongest correlation involving crustal elements was between IL-6 release and the aluminum:silicon ratio. The observed correlations between low-volatility organic components of soil- and road-derived dusts and the cytokine release by BEAS-2B cells are relevant for investigation of mechanisms linking specific air pollution particle types with the initiating events leading to airway inflammation in sensitive populations. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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