Measurement of antinuclear antibodies by multiplex immunoassay: a prospective, multicenter clinical evaluation.
OBJECTIVE: We conducted a prospective, multicenter evaluation of autoantibody testing by multiplex immunoassay in patients with known or suspected connective tissue diseases (CTD). We evaluated agreement between multiplex immunoassay and enzyme immunoassay (EIA) and assessed the diagnostic utility o...
| Publicado en: | Journal of Rheumatology Vol. 34; no. 5; pp. 978 - 987 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Journal of Rheumatology Publishing Co. Ltd.
May2007
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106151528&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106151528 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0315162X 4CM jtl: Journal of Rheumatology issn: 0315162X maglogo: N pubinfo: dt: May2007 vid: 34 iid: 5 pid: 6803 pub: Journal of Rheumatology Publishing Co. Ltd. place: Toronto, Ontario artinfo: ui: 106151528 106151528 2009609751 NLM17407218 106151528 ppf: 978 ppct: 9 formats: tig: atl: Measurement of antinuclear antibodies by multiplex immunoassay: a prospective, multicenter clinical evaluation. aug: au: Moder KG Wener MH Weisman MH Ishimori ML Wallace DJ Buckeridge DL Homburger HA affil: Department of Rheumatology, Mayo College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA. sug: subj: Autoantibodies Blood Connective Tissue Diseases Diagnosis Immunoassay Methods Adolescence Adult Aged Aged, 80 and Over Algorithms Confidence Intervals Connective Tissue Diseases Immunology Descriptive Statistics Diagnosis, Computer Assisted Female Funding Source Information Science kappa Statistic Male Middle Age Reproducibility of Results Human Adolescent: 13-18 years Adult: 19-44 years Aged: 65+ years Aged, 80 & over Middle Aged: 45-64 years Female Male ab: OBJECTIVE: We conducted a prospective, multicenter evaluation of autoantibody testing by multiplex immunoassay in patients with known or suspected connective tissue diseases (CTD). We evaluated agreement between multiplex immunoassay and enzyme immunoassay (EIA) and assessed the diagnostic utility of autoantibody profiles. METHODS: Samples from 908 patients with suspected CTD seen in rheumatology clinics were collected prospectively at 3 tertiary care centers. Diagnoses were established according to recognized classification criteria. Tests for autoantibodies were obtained by multiplex immunoassay and by EIA. The results of the multiplex immunoassay were analyzed using a previously validated interpretative algorithm, MDSS (Medical Decision Support Software), that suggests possible disease associations based on the pattern of results for the autoantibodies. RESULTS: The median patient age was 49.7 years; 83% were female. The most common diagnoses were rheumatoid arthritis in 352 patients and systemic lupus erythematosus (SLE) in 332 patients. Agreement between multiplex and EIA testing ranged from a high of 99% (95% CI 98% to 100%) for Jo-1 to a low of 79% (95% CI 76% to 82%) for antinuclear antibodies. The MDSS algorithm suggested an appropriate disease association in 75% to 100% of patients with SLE. The results varied depending on the disease and the autoantibodies present. CONCLUSION: These results suggest that patterns of autoantibodies detected by multiplex immunoassay testing, when analyzed by an interpretative algorithm, are useful in the evaluation of patients with CTD in situations of high disease prevalence. Further testing is necessary to determine its utility in settings of low disease prevalence. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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