Fecal water as a non-invasive biomarker in nutritional intervention: comparison of preparation methods and refinement of different endpoints.
The assessment of cellular effects by the aqueous phase of human feces (fecal water, FW) is a useful biomarker approach to study cancer risks and protective activities of food. In order to refine and develop the biomarker, different protocols of preparing FW were compared. Fecal waters were prepared...
| Publicado en: | Nutrition & Cancer Vol. 57; no. 2; pp. 158 - 168 |
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| Autores principales: | , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2007
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106164368&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106164368 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2007 vid: 57 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 106164368 106164368 2009623828 10.1080/01635580701274848 NLM17571949 106164368 ppf: 158 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Fecal water as a non-invasive biomarker in nutritional intervention: comparison of preparation methods and refinement of different endpoints. aug: au: Klinder A Karlsson PC Clune Y Hughes R Glei M Rafter JJ Rowland I Collins JK Pool-Zobel BL affil: Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University, Jena, Germany. sug: subj: Diagnosis, Laboratory Standards Feces Analysis Animal Studies Biological Markers Centrifugation Methods Colonic Neoplasms Epidemiology Comparative Studies Data Analysis Software DNA Electrophoresis Methods Electrophoresis, Agar Gel Evaluation Research Funding Source In Vitro Studies Neoplasms Epidemiology One-Way Analysis of Variance Paired T-Tests Risk Assessment Tissue Culture Techniques Water Utilization ab: The assessment of cellular effects by the aqueous phase of human feces (fecal water, FW) is a useful biomarker approach to study cancer risks and protective activities of food. In order to refine and develop the biomarker, different protocols of preparing FW were compared. Fecal waters were prepared by 3 methods: (A) direct centrifugation; (B) extraction of feces in PBS before centrifugation; and (C) centrifugation of lyophilized and reconstituted feces. Genotoxicity was determined in colon cells using the Comet assay. Selected samples were investigated for additional parameters related to carcinogenesis. Two of 7 FWs obtained by methods A and B were similarly genotoxic. Method B, however, yielded higher volumes of FW, allowing sterile filtration for long-term culture experiments. Four of 7 samples were non-genotoxic when prepared according to all 3 methods. FW from lyophilized feces and from fresh samples were equally genotoxic. FWs modulated cytotoxicity, paracellular permeability, and invasion, independent of their genotoxicity. All 3 methods of FW preparation can be used to assess genotoxicity. The higher volumes of FW obtained by preparation method B greatly enhance the perspectives of measuring different types of biological parameters and using these to disclose activities related to cancer development. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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