Detection of hypopharyngeal squamous cell carcinoma using serum proteomics.
Conclusions. The combination of surface-enhanced laser desorption/ionization (SELDI) with bioinformatics tools could help find serum proteome biomarkers and establish a predictive model for early detection of hypopharyngeal squamous cell carcinoma (HSCC). Objectives. Proteomic profiling of serum usi...
| Publicado en: | Acta Oto-Laryngologica Vol. 126; no. 8; pp. 853 - 861 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts tracings Journal Article |
| Publicado: |
Taylor & Francis Ltd
Aug2006
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106232247&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106232247 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Aug2006 vid: 126 iid: 8 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 106232247 2009265892 10.1080/00016480500525205 NLM16846929 106232247 ppf: 853 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Detection of hypopharyngeal squamous cell carcinoma using serum proteomics. aug: au: Zhou L Cheng L Tao L Jia X Lu Y Liao P affil: Department of Otorlaryngology-Head and Neck Surgery, Fudan University Affiliated Eye, Ear, Nose and Throat Hospital, 83 FenYang Road, 200031 Shanghai, China; zhlwc@online.sh.cn sug: subj: Carcinoma, Squamous Cell Diagnosis Pharyngeal Neoplasms Diagnosis Proteomics Utilization Adult Aged Aged, 80 and Over Algorithms Blood Proteins Blood Analysis Case Control Studies Decision Trees Descriptive Statistics Funding Source Middle Age Sensitivity and Specificity Human Adult: 19-44 years Aged: 65+ years Aged, 80 & over Middle Aged: 45-64 years ab: Conclusions. The combination of surface-enhanced laser desorption/ionization (SELDI) with bioinformatics tools could help find serum proteome biomarkers and establish a predictive model for early detection of hypopharyngeal squamous cell carcinoma (HSCC). Objectives. Proteomic profiling of serum using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is an emerging technique to identify new biomarkers in biological fluids and to establish clinically useful diagnostic computational models. We used it to find new potential biomarkers and to establish a predictive model for early detection of HSCC. Materials and methods. One hundred serum samples including 48 from HSCC patients and 52 from normal controls which were divided into a training set and a blind testing set were treated on WCX2 and IMAC3 protein chip, and serum protein or peptide patterns were detected by SELDI-TOF-MS. The data of spectra were analyzed by Biomarker Wizard software to screen serum proteome biomarkers of HSCC. A decision tree classification algorithm and blind validation were determined by Biomarker Pattern Software (BPS). Results. Ranging from 2 to 30 kDa, 45 potential biomarkers could differentiate HSCC patients from normal controls (p <0.05). Among them four candidate protein peaks with m/z values of 7796, 4216, 5927, and 5361Da were selected to establish a predictive model by BPS with sensitivity of 94% and specificity of 89%. A sensitivity of 92% and specificity of 82% were validated in the blind testing set. pubtype: Academic Journal doctype: research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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