Equol: a comparison of the effects of the racemic compound with that of the purified S-enantiomer on the growth, invasion, and DNA integrity of breast and prostate cells in vitro.
It has been postulated that the R- and S-equol enantiomers have different biological properties given their different binding affinities for the estrogen receptor. S-(-)equol is produced via the bacterial conversion of the soy isoflavone daidzein in the gut. We have compared the biological effects o...
| Publicado en: | Nutrition & Cancer Vol. 54; no. 2; pp. 232 - 243 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2006
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106294347&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106294347 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2006 vid: 54 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 106294347 106294347 2009293457 10.1207/s15327914nc5402_10 NLM16898868 106294347 ppf: 232 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Equol: a comparison of the effects of the racemic compound with that of the purified S-enantiomer on the growth, invasion, and DNA integrity of breast and prostate cells in vitro. aug: au: Magee PJ Raschke M Steiner C Duffin JG Pool-Zobel BL Jokela T Wahala K Rowland IR affil: Northern Ireland Centre for Food and Health, University of Ulster, Coleraine, Northern Ireland. pj.magee@ulster.ac.uk sug: subj: Breast Neoplasms Pathology DNA Drug Effects Isoflavones Pharmacodynamics Phytoestrogens Pharmacodynamics Prostatic Neoplasms Pathology Aged Cell Physiology Drug Effects Data Analysis Software DNA Metabolism Dose-Response Relationship, Drug Electrophoresis, Agar Gel Female Funding Source Gene Expression Drug Effects In Vitro Studies Isoflavones Analysis Male Middle Age One-Way Analysis of Variance Phytoestrogens Analysis Post Hoc Analysis Receptors, Cell Surface Metabolism T-Tests Tissue Culture Techniques Human Aged: 65+ years Middle Aged: 45-64 years Female Male ab: It has been postulated that the R- and S-equol enantiomers have different biological properties given their different binding affinities for the estrogen receptor. S-(-)equol is produced via the bacterial conversion of the soy isoflavone daidzein in the gut. We have compared the biological effects of purified S-equol to that of racemic (R and S) equol on breast and prostate cancer cells of varying receptor status in vitro. Both racemic and S-equol inhibited the growth of the breast cancer cell line MDA-MB-231 (> or = 10 microM) and the prostate cancer cell lines LNCaP (> or = 5 microM) and LAPC-4 (> or = 2.5 microM). The compounds also showed equipotent effects in inhibiting the invasion of MDA-MB-231 and PC-3 cancer cells through matrigel. S-equol (1, 10, 30 microM) was unable to prevent DNA damage in MCF-7 or MCF-10A breast cells following exposure to 2-hydroxy-4-nonenal, menadione, or benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide. In contrast, racemic equol (10, 30 microM) prevented DNA damage in MCF-10A cells following exposure to 2-hydroxy-4-nonenal or menadione. These findings suggest that racemic equol has strong antigenotoxic activity in contrast to the purified S-equol enantiomer implicating the R-, rather than the S-enantiomer as being responsible for the antioxidant effects of equol, a finding that may have implications for the in vivo chemoprotective properties of equol. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|