Implantation of perichondrium-derived chondrocytes in physeal defects of rabbit tibiae.
BACKGROUND: Various cells and scaffolds have been used experimentally as interposition materials after physeal bar resection to treat partial physeal growth arrest. However, results of these cell therapies are unpredictable. METHODS: We investigated the chondrogenic differentiation of rabbit pericho...
| Publicado en: | Acta Orthopaedica Vol. 76; no. 5; pp. 628 - 637 |
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| Autores principales: | , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Medical Journals Sweden AB
Oct2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106313131&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106313131 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17453674 1BVL jtl: Acta Orthopaedica issn: 17453674 maglogo: N pubinfo: dt: Oct2005 vid: 76 iid: 5 pid: 59195 pub: Medical Journals Sweden AB artinfo: ui: 106313131 2009234591 10.1080/17453670510041691 NLM16263608 106313131 ppf: 628 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Implantation of perichondrium-derived chondrocytes in physeal defects of rabbit tibiae. aug: au: Yoo WJ Choi IH Chung CY Cho T Kim I Kim CJ affil: Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea. sug: subj: Connective Tissue Cells Transplantation Tibia Surgery Analysis of Variance Animal Studies Cells Descriptive Statistics Fibrin Metabolism Funding Source Immunohistochemistry Osteogenesis Rabbits Repeated Measures T-Tests Tibia Metabolism Tibia Pathology ab: BACKGROUND: Various cells and scaffolds have been used experimentally as interposition materials after physeal bar resection to treat partial physeal growth arrest. However, results of these cell therapies are unpredictable. METHODS: We investigated the chondrogenic differentiation of rabbit perichondrial cells in vitro. We also investigated radiographic and histological changes after implantation of the perichondrium-derived chondrocytes, embedded in fibrin beads, into defects created in the medial aspect of the proximal tibial physis of 26 8-week-old New Zealand White rabbits. Physeal defects of the contralateral tibiae were left intact without any treatment, or were treated with fibrin beads not containing cells. RESULTS: The perichondrial cells cultured in the alginate- fibrin beads were positive in immunohistochemical staining for S-100 protein and the extracellular area was stained with Safranin-O. RNase protection assay showed that the expression of type II collagen and aggrecan continued In the cell and bead-treated tibiae, varus deformity was reduced significantly and tibial length was maintained longer than that of the contralateral tibia. Histologically, cartilage clusters of varying size with intervening micro-bony trabeculae were found in the cell and bead-treated tibiae. The lateral, remaining physis in the untreated tibiae showed altered histology. INTERPRETATION: Implantation of fibrin beads containing perichondrium-derived chondrocytes into the physeal defect helps to prevent angular deformation and shortening of long bone, but not completely. Physeal bony bar formation adversely affects the growth of the remaining physis from early stages onward. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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