Antigen microarray profiling of autoantibodies in rheumatoid arthritis.
OBJECTIVE: Because rheumatoid arthritis (RA) is a heterogeneous autoimmune disease in terms of disease manifestations, clinical outcomes, and therapeutic responses, we developed and applied a novel antigen microarray technology to identify distinct serum antibody profiles in patients with RA. METHOD...
| Publicado en: | Arthritis & Rheumatism Vol. 52; no. 9; pp. 2645 - 2656 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106333828&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106333828 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00043591 04N jtl: Arthritis & Rheumatism issn: 00043591 maglogo: Y pubinfo: dt: Sep2005 vid: 52 iid: 9 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 106333828 2009271822 10.1002/art.21269 NLM16142722 106333828 ppf: 2645 ppct: 11 formats: tig: atl: Antigen microarray profiling of autoantibodies in rheumatoid arthritis. aug: au: Hueber W Kidd BA Tomooka BH Lee BJ Bruce B Fries JF Sønderstrup G Monach P Drijfhout JW van Venrooij WJ Utz PJ Genovese MC Robinson WH affil: Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Palo Alto VA Health Care System, MC 154R, 3801 Miranda Avenue, Palo Alto, CA 94304; whueber@stanford.edu sug: subj: Antigens Blood Arthritis, Rheumatoid Immunology Autoantibodies Blood Adult Aged Arthritis, Rheumatoid Physiopathology California Case Control Studies Clinical Assessment Tools Data Analysis, Statistical Descriptive Statistics Female Health Status Indicators In Vitro Studies Male Middle Age Netherlands Questionnaires Funding Source Human Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: OBJECTIVE: Because rheumatoid arthritis (RA) is a heterogeneous autoimmune disease in terms of disease manifestations, clinical outcomes, and therapeutic responses, we developed and applied a novel antigen microarray technology to identify distinct serum antibody profiles in patients with RA. METHODS: Synovial proteome microarrays, containing 225 peptides and proteins that represent candidate and control antigens, were developed. These arrays were used to profile autoantibodies in randomly selected sera from 2 different cohorts of patients: the Stanford Arthritis Center inception cohort, comprising 18 patients with established RA and 38 controls, and the Arthritis, Rheumatism, and Aging Medical Information System cohort, comprising 58 patients with a clinical diagnosis of RA of <6 months duration. Data were analyzed using the significance analysis of microarrays algorithm, the prediction analysis of microarrays algorithm, and Cluster software. RESULTS: Antigen microarrays demonstrated that autoreactive B cell responses targeting citrullinated epitopes were present in a subset of patients with early RA with features predictive of the development of severe RA. In contrast, autoimmune targeting of the native epitopes contained on synovial arrays, including several human cartilage gp39 peptides and type II collagen, were associated with features predictive of less severe RA. CONCLUSION: Proteomic analysis of autoantibody reactivities provides diagnostic information and allows stratification of patients with early RA into clinically relevant disease subsets. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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