Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction.
An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles steph...
| Publicado en: | Military Medicine Vol. 170; no. 12; pp. 1060 - 1066 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Dec2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106412438&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106412438 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00264075 4DV jtl: Military Medicine issn: 00264075 maglogo: N pubinfo: dt: Dec2005 vid: 170 iid: 12 pid: 622 pub: Oxford University Press / USA artinfo: ui: 106412438 106412438 NLM16491948 2009116685 10.7205/milmed.170.12.1060 NLM16491948 106412438 ppf: 1060 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction. aug: au: McAvin JC Bowles DE Swaby JA Blount KW Blow JA Quintana M Hickman JR Atchley DH Niemeyer DM McAvin, James C Bowles, David E Swaby, James A Blount, Keith W Blow, Jamie A Quintana, Miguel Hickman, John R Atchley, Daniel H Niemeyer, Debra M affil: Epidemiological Surveillance Division, U.S. Air Force Institute for Environment Safety, San Antonio, TX 78235-5237, USA sug: subj: Mosquitoes Classification Polymerase Chain Reaction Analytic Research False Positive Results Honduras In Vitro Studies Sensitivity and Specificity Statistical Significance Human ab: An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles stephensi, and Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demonstrated 100% concordance in both in vitro sensitivity (six of six samples) and specificity (10 of 10 samples). A single adult A. aegypti was identified in a pool of 100 non-A. aegypti mosquitoes. The limit of detection of A. aegypti egg pools was five individual eggs. Field testing was conducted in central Honduras. An A. aegypti and Culex spp. panel of individual and mixed pools (n = 30) of adult mosquitoes, pupae, and larvae demonstrated 100% concordance in sensitivity (22 of 22 samples) and 97% concordance in specificity (29 of 30 samples), with one false-positive result. Field testing of an A. aegypti and Culex spp. blind panel (n = 16) consisting of individual and mixed pools of adult mosquitoes, pupae, and larvae demonstrated 90% concordance in sensitivity (nine of 10 samples) and 88% concordance in specificity (14 of 16 samples). pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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