The potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis.
BACKGROUND: Both rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available. OBJECTIVE: The objective of this study was to evaluate the levels of antibodies agai...
| Publicado en: | JRC: Journal of Clinical Rheumatology Vol. 12; no. 1; pp. 11 - 17 |
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| Autores principales: | , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Feb2006
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106432854&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106432854 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10761608 N16 jtl: JRC: Journal of Clinical Rheumatology issn: 10761608 maglogo: N pubinfo: dt: Feb2006 vid: 12 iid: 1 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 106432854 106432854 2009151973 10.1097/01.rhu.0000200374.14619.f2 NLM16484874 106432854 ppf: 11 ppct: 6 formats: tig: atl: The potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis. aug: au: Rashid T Ebringer A Wilson C Bansal S Paimela L Binder A affil: Division of Health and Life Sciences, King's College London, UK sug: subj: Antibodies Diagnostic Use Arthritis, Rheumatoid Diagnosis Spondylitis, Ankylosing Diagnosis Adult Aged Confidence Intervals Enzyme-Linked Immunosorbent Assay Female Male Middle Age Sensitivity and Specificity T-Tests United Kingdom Funding Source Human Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: BACKGROUND: Both rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available. OBJECTIVE: The objective of this study was to evaluate the levels of antibodies against Proteus and Klebsiella antigenic peptides in an endeavor to develop diagnostic indices for the identification of patients with RA and AS, respectively. METHODS: Sera from 50 patients with RA, 34 patients with AS, and 38 healthy subjects were screened for antibodies against 'ESRRAL' and 'IRRET' synthetic amino acid peptides obtained from Proteus hemolysin and urease (HU) as well as against 'QTDRED' and 'DRDE' peptides from Klebsiella nitrogenase and pullulanase (NP) proteins, respectively. Multiplication of the 2 antibodies against each organism produced indices for RA-HU and AS-NP. RESULTS: Significantly increased levels of anti-HU antibodies (P<0.0001) were observed in patients with RA when compared with patients with AS or with healthy control subjects. Patients with AS were found to have significantly elevated levels of anti-NP (P<0.0001) antibodies when compared with patients with RA or with healthy subjects. Furthermore, all patients with RA were found to have values of anti-HU antibody (RA-HU) index above 95% confidence limit (CL) of the mean of healthy control subjects; meanwhile, all patients with AS were having values of anti-NP antibody (AS-NP) index above the 95% CL of the mean of healthy control subjects (100% sensitivity). However, the specificity of the RA-HU index in RA and the AS-NP index in patients with AS were 92% and 95%, respectively. CONCLUSION: The use of the RA-HU or AS-NP diagnostic index as a sole marker or in combination with other autoantibody markers could be used in the identification of patients with RA or AS, respectively. Longitudinal investigations starting with patients with early disease will be needed. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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