Does Helicobacter pylori colonize the nasopharynx of children and contribute to their middle ear disease?
OBJECTIVE: There is growing interest in studying the presence of HP in the upper aerodigestive tract. It was shown in several pilot studies that it colonizes the area, while other authors found no evidence of its presence there and a third group of authors believed that it had only a transient prese...
| Publicado en: | Acta Oto-Laryngologica Vol. 126; no. 2; pp. 154 - 160 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Feb2006
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106456868&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106456868 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Feb2006 vid: 126 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 106456868 106456868 2009185034 10.1080/00016480500312679 NLM16428192 106456868 ppf: 154 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Does Helicobacter pylori colonize the nasopharynx of children and contribute to their middle ear disease? aug: au: Bitar M Mahfouz R Soweid A Racoubian E Ghasham M Zaatari G Fuleihan N affil: Department of Otolaryngology-Head & Neck Surgery, American University of Beirut Medical Center, PO Box 11-0236/A52, Beirut 1107-2020, Lebanon; mb36@aub.edu.lb sug: subj: Bacterial Colonization In Infancy and Childhood Helicobacter Pylori Otitis Media Etiology Adenoids Child Child, Preschool Descriptive Statistics Funding Source Gastroesophageal Reflux Complications Nose Otitis Media with Effusion Pharynx Polymerase Chain Reaction Human Child: 6-12 years Child, Preschool: 2-5 years ab: OBJECTIVE: There is growing interest in studying the presence of HP in the upper aerodigestive tract. It was shown in several pilot studies that it colonizes the area, while other authors found no evidence of its presence there and a third group of authors believed that it had only a transient presence there. In this study we investigated a possible role for HP in middle ear disease in children. MATERIAL AND METHODS: Consecutive patients undergoing myringotomy and adenoidectomy for chronic otitis media with effusion or recurrent otitis media were enrolled. Middle ear fluids were cultured on three types of agar plate (Brucella + laked horse blood; Brucella + sheep blood; and chocolate). A double polymerase chain reaction (PCR) was run to detect urease-C and adhesion subunit genes. Rapid urease enzyme testing and PCR were used on the adenoid specimens. Parents were interviewed regarding symptoms suggestive of gastroesophageal reflux in their children. RESULTS: Eighteen patients were enrolled in the study (mean age 4.4 years; age range 3-8 years) with an equal gender distribution. All 28 middle ear fluid cultures were negative in all 3 media. Twenty-one of the 28 samples contained DNA, yet PCR revealed that none of them belonged to HP. Ten of the 13 adenoid specimens obtained were positive on rapid urease testing, but none on PCR. Seven of the 18 patients had at least 1 symptom suggestive of gastroesophageal reflux during the 6 months preceding the study but this did not have an impact on any of the results. CONCLUSION: There was no evidence from this study that Helicobacter pylori (HP) colonizes the nasopharynx of children with middle ear disease, whether dyspeptic or not. There is also no apparent role for this bacterium in middle ear pathology. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|