Is cervical dilatation during parturition at term associated with apoptosis?
AIMS: Cellular turnover may be involved in remodeling of the cervix during parturition. Therefore, the number and localization of apoptotic and proliferating cells during cervical dilatation at term were determined. METHODS: Biopsy specimens from the lower uterine segment of 36 women undergoing cesa...
| Publicado en: | Journal of Perinatal Medicine Vol. 33; no. 2; pp. 137 - 144 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
De Gruyter
2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106469626&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106469626 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03005577 0FP jtl: Journal of Perinatal Medicine issn: 03005577 maglogo: N pubinfo: dt: 2005 vid: 33 iid: 2 pid: 1734 pub: De Gruyter place: , <Blank> artinfo: ui: 106469626 106469626 2009208447 10.1515/jpm.2005.026 NLM15843264 106469626 ppf: 137 ppct: 7 formats: tig: atl: Is cervical dilatation during parturition at term associated with apoptosis? aug: au: Kemp B Rath W Winkler M Reineke T Beier HM von Rango U affil: Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany; dr.kemp@t-online.de sug: subj: Apoptosis Cervix Dilatation and Effacement Physiology Labor Physiology Adult Birth Weight Cell Death Cervix Anatomy and Histology Cesarean Section Confidence Intervals Female Gestational Age Immunohistochemistry Infant, Newborn Male Poisson Distribution Power Analysis Pregnancy Pregnancy Outcomes Human Adult: 19-44 years Infant, Newborn: birth-1 month Female Male ab: AIMS: Cellular turnover may be involved in remodeling of the cervix during parturition. Therefore, the number and localization of apoptotic and proliferating cells during cervical dilatation at term were determined. METHODS: Biopsy specimens from the lower uterine segment of 36 women undergoing cesarean section with a cervical dilatation of < 2 cm (n = 10), 2- < 4 cm (n = 9), 4-6 cm (n = 8), and > 6 cm (n = 9) were examined for nuclear fragmentation by the TUNEL assay, and for cell survival by the apoptosis-blocking bcl-2. Proliferation was marked by Ki-67, epithelial cells by cytokeratin and leukocytes by CD 45. For quantification of apoptotic and proliferating cells, eight random fields of each specimen stained for TUNEL or Ki-67 were blindly counted by two investigators. For statistical evaluation, 90% confidence intervals based on a Poisson distribution were used; groups with non-overlapping intervals were considered significantly different. RESULTS: Apoptotic cells were found exclusively within the stromal compartment, while bcl-2 was expressed in epithelial cells and leukocytes. Proliferating cells were of stromal and epithelial origin. The number of apoptotic as well as proliferating cells ranged from 0 to 2 cells per high-power field (median number 0) in all groups. The confidence intervals were overlapping for all groups, showing no statistical difference between them. CONCLUSION: Apoptosis does not seem to play a decisive role in the process of cervical dilatation during parturition at term. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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