Identification of Helicobacter pylori DNA in Iranian patients with gallstones.
In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobact...
| Publicado en: | Epidemiology & Infection Vol. 132; no. 6; pp. 1185 - 1190 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | pictorial research Journal Article |
| Publicado: |
Cambridge University Press
Dec2004
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106579873&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106579873 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09502688 2S0 jtl: Epidemiology & Infection issn: 09502688 maglogo: N pubinfo: dt: Dec2004 vid: 132 iid: 6 pid: 15979 pub: Cambridge University Press artinfo: ui: 106579873 106579873 NLM15635979 2005035524 10.1017/s0950268804002985 NLM15635979 106579873 ppf: 1185 ppct: 5 formats: tig: atl: Identification of Helicobacter pylori DNA in Iranian patients with gallstones. aug: au: Farshad SH Alborzi A Hosseini SAM Oboodi B Rasouli M Japoni A Nasiri J Farshad, Sh Alborzi, A Malek Hosseini, S A Oboodi, B Rasouli, M Japoni, A Nasiri, J affil: Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran sug: subj: Cholelithiasis DNA Iran Helicobacter Infections Familial and Genetic Adult Aged Funding Source Iran Middle Age Polymerase Chain Reaction Human Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years ab: In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobacter genus and H. pylori respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (control group). In 18.1% of stone and 12.1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and culture tests were negative for all samples. The PCR was negative in the control group. In conclusion, H. pylori DNA was detected in stone samples of Iranian patients with gallstones but we are not sure of their viability. To clarify the clinical role of Helicobacter in gallbladder diseases, studies using accurate tests on larger patient and control groups are needed to ascertain whether this microorganism is an innocent bystander or active participant in gallstone formation. pubtype: Academic Journal doctype: pictorial research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|