Stem-cell consequences of embryo epigenetic defects.
Context: The genetic code in the DNA of virtually every somatic cell can produce the entire complement of encoded proteins. Acetylation of histones and methylation of histones and DNA cytosine residues are part of the complex epigenetic regulatory process determining lineage-specific gene expression...
| Publicado en: | Lancet Vol. 364; no. 9429; pp. 206 - 209 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial review Journal Article |
| Publicado: |
Lancet
7/10/2004
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106583513&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106583513 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01406736 LAN jtl: Lancet issn: 01406736 maglogo: N pubinfo: dt: 7/10/2004 vid: 364 iid: 9429 pid: 1297 pub: Lancet place: Philadelphia, Pennsylvania artinfo: ui: 106583513 106583513 NLM15246736 2005039278 10.1016/s0140-6736(04)16636-1 NLM15246736 106583513 ppf: 206 ppct: 3 formats: fmt: @attributes: type: P tig: atl: Stem-cell consequences of embryo epigenetic defects. aug: au: Allegrucci C Denning C Priddle H Young L Allegrucci, Cinzia Denning, Chris Priddle, Helen Young, Lorraine affil: Division of Obstetrics and Gynaecology, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK sug: subj: Embryo Physiology Fetal Development Genetics Stem Cells Physiology DNA Embryology Genetic Techniques Stem Cells Transplantation ab: Context: The genetic code in the DNA of virtually every somatic cell can produce the entire complement of encoded proteins. Acetylation of histones and methylation of histones and DNA cytosine residues are part of the complex epigenetic regulatory process determining lineage-specific gene expression by altering the local structure of chromatin. After fertilisation, sperm DNA exchanges protamines for histones recruited from oocyte cytoplasm, reconfiguring both parental genomes into an epigenetic state conducive to activating the embryonic developmental programme. The identification of epigenetic reprogramming mechanisms is a major interest, rekindled by the ability of at least some somatic cells to acquire totipotency after somatic-cell nuclear transfer.Starting Point: Recently, Woo SukHwang and colleagues (Science 2004; 303: 1669-74) derived a human embryonic stem-cell line from embryo therapeutic cloning. Chad Cowan and colleagues (N Engl JMed 2004; 350: 1353-56) produced 17 new lines from embryos supernumerary to infertility treatments. However, increasing evidence from a range of mammals shows a propensity for epigenetic errors with embryo technologies. If paralleled in human embryos, the effect on tumorigenic and differentiation properties of embryonic stem cells needs to be established. WHERE NEXT? Identifying the mechanisms in the oocyte that reprogramme a somatic cell to the embryonic state might allow somatic cells to be reprogrammed ex ovo by in-vitro manipulation of the epigenome. Because the oocyte is designed to reprogramme the sperm genome, which is in a different chromatin state from a somatic cell, perhaps many of the epigenetic errors induced by somatic-cell nuclear transfer could be avoided by a more targeted approach. pubtype: Academic Journal doctype: pictorial review Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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