Detection of dientamoeba fragilis by culture...reprinted from the British Journal of Biomedical Sciences May 2003 with permission of the publisher

Symptoms associated with Dientamoeba fragilis include diarrhoea, abdominal pain, nausea, vomiting, epigastric pain and weight loss. A possible link between D. fragilis and irritable bowel syndrome (IBS)-like symptoms has been reported, and therefore the presence of this parasite should be excluded b...

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Publicado en:Journal of Continuing Education Topics & Issues Vol. 6; no. 3; pp. 144 - 150
Autores principales: Windsor JJ, MacFarlane L, Hughes-Thapa G, Jones SKA, Whiteside TM
Formato: CEU exam questions pictorial tables/charts Journal Article
Publicado: American Medical Technologists 2004 Aug
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2004 Aug
      vid: 6
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      pub: American Medical Technologists
      place: Rosemont, Illinois
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        atl: Detection of dientamoeba fragilis by culture...reprinted from the British Journal of Biomedical Sciences May 2003 with permission of the publisher
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        au:
          Windsor JJ
          MacFarlane L
          Hughes-Thapa G
          Jones SKA
          Whiteside TM
        affil: MPHS Microbiology Aberystwyth, Bronglais Hospital, Aberystwyth, Ceredigion, UK
      sug:
        subj:
          Amebiasis Diagnosis
          Diagnosis, Laboratory
          Education, Continuing (Credit)
      ab: Symptoms associated with Dientamoeba fragilis include diarrhoea, abdominal pain, nausea, vomiting, epigastric pain and weight loss. A possible link between D. fragilis and irritable bowel syndrome (IBS)-like symptoms has been reported, and therefore the presence of this parasite should be excluded before making a diagnosis of IBS. Over a six-month period, 976 faecal samples were submitted to NPHS Microbiology Aberystwyth for routine microbiological analysis. All samples were also cultured for parasites using Robinson's xenic medium. Trichrome staining was undertaken whenever practicable, but many stools had insufficient material. D. fragilis was isolated from 25 (2.6%) patients, whereas Cryptosporidium spp. was detected in 16 (1.6%) patients. D. fragilis was only detected in nine (1.3%) out of 685 specimens stained with trichrome, although four of the 25 culture-positive stools had insufficient sample for staining. Parasite culture proved to be less laborious than trichrome staining and dramatically increased D. fragilis detection rate.
      pubtype: Academic Journal
      doctype:
        CEU
        exam questions
        pictorial
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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