Helicobacter pylori infection and infertility.
Objective: To determine (1) the prevalence of Helicobacter pylori infection in male and female patients with reproductive disorders and controls; (2) the presence of anti-H. pylori antibodies in samples of follicular fluid, vaginal secretions and sperm; and (3) the existence of a structural homology...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 14; no. 6; pp. 663 - 670 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
2002 Jun
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106817970&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106817970 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: 2002 Jun vid: 14 iid: 6 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 106817970 106817970 NLM12072601 2003046557 10.1097/00042737-200206000-00012 NLM12072601 106817970 ppf: 663 ppct: 7 formats: tig: atl: Helicobacter pylori infection and infertility. aug: au: Figura N Piomboni P Ponzetto A Gambera L Lenzi C Vaira D Peris C Lotano MR Gennari L Bianciardi L Renieri T Valensin PE Capitani S Moretti E Colapinto R Baccetti B Gennari C Figura, N Piomboni, P Ponzetto, A affil: Institute of Internal Medicine, University of Sienna, Sienna, Italy sug: subj: Helicobacter Infections Complications Infertility Etiology Antibodies, Bacterial Analysis Blotting, Western Enzyme-Linked Immunosorbent Assay Helicobacter Pylori Immunology Spermatozoa Case Control Studies T-Tests Adolescence Adult Middle Age Female Funding Source Human Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Female ab: Objective: To determine (1) the prevalence of Helicobacter pylori infection in male and female patients with reproductive disorders and controls; (2) the presence of anti-H. pylori antibodies in samples of follicular fluid, vaginal secretions and sperm; and (3) the existence of a structural homology between a major spermatozoa protein, tubulin, and H. pylori proteins.Patients and Methods: Serum samples from 167 patients with infertility and 837 age- and gender-matched controls (blood donors) were examined by enzyme-linked immunosorbent assay (ELISA) and Western blotting to determine the seropositivity for H. pylori infection. The presence of anti-H. pylori antibodies in samples of follicular fluid, vaginal secretions and sperm was determined using the same techniques. The possible cross-reactivity with spermatozoa of anti-H. pylori hyperimmune sera and human antibodies was studied by immunofluorescence. The N-acid homology of human tubulin with the principal H. pylori proteins was assayed by the WU-blastp program available on the Internet.Results: The prevalence of infection was significantly higher in patients than controls (49.1% v. 33.5%, P < 0.001). Follicular fluids from infected patients contained specific antibodies in all cases, sperm samples in about 50% of cases, and vaginal secretions in a minority of cases. Sera to H. pylori whole antigens and VacA reacted with the tails and the pericentriolar area of human spermatozoa (which are rich in tubulin); sera to urease and heat-shock protein (Hsp) did not. Follicular fluids with anti-H. pylori antibodies immune reacted with spermatozoa. A linear homology was found between beta-tubulin and three H. pylori proteins, flagellin, VacA and CagA.Conclusions: H. pylori infection may increase the risk of developing reproductive disorders or worsen the clinical expression of this syndrome. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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