Utility and limitations of a method for detecting Helicobacter pylori-specific antigens in the stool.

BACKGROUND: Recently, a method for detecting Helicobacter pylori-specific antigens in the stool (HpSA) has been proposed to be useful clinically. The aim of this study was to determine the accuracy of HpSA for the diagnosis of H. pylori infection, including early assessment after eradication treatme...

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Publicado en:Journal of Gastroenterology Vol. 38; no. 3; pp. 222 - 229
Autores principales: Matsuda M, Noda Y, Takemori Y
Formato: research tables/charts Journal Article
Publicado: Springer Nature 2003
Acceso en línea:Ver este registro en EBSCOhost
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        09441174
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      dt: 2003
      vid: 38
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      pub: Springer Nature
      place: New York, New York
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        106876119
        2003135353
        10.1007/s005350300040
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        106876119
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        atl: Utility and limitations of a method for detecting Helicobacter pylori-specific antigens in the stool.
      aug:
        au:
          Matsuda M
          Noda Y
          Takemori Y
        affil: Dept of Internal Medicine, Toyama Rosai Hospital, Uozu, Japan
      sug:
        subj:
          Antigens Analysis
          Feces Analysis
          Helicobacter Infections Diagnosis
          Adult
          Aged, 80 and Over
          Biopsy
          Descriptive Statistics
          Endoscopy
          Female
          Helicobacter Pylori Drug Effects
          Immunoassay
          Japan
          Male
          Mann-Whitney U Test
          Middle Age
          Prospective Studies
          Sensitivity and Specificity
          Spearman's Rank Correlation Coefficient
          Wilcoxon Signed Rank Test
          Human
          Adult: 19-44 years
          Aged, 80 & over
          Middle Aged: 45-64 years
          Female
          Male
      ab: BACKGROUND: Recently, a method for detecting Helicobacter pylori-specific antigens in the stool (HpSA) has been proposed to be useful clinically. The aim of this study was to determine the accuracy of HpSA for the diagnosis of H. pylori infection, including early assessment after eradication treatment, and the potential for quantitative evaluation of H. pylori in the stomach. METHODS: The subjects were 85 patients with gastroduodenal disorders who underwent endoscopic examination. Of these, 36 were treated for eradication of H. pylori infection and reassessed 4-8 weeks after treatment. HpSA was tested by enzyme immunoassay. For the definitive diagnosis of H. pylori infection, biopsy specimens were taken endoscopically and examined by quantitative culture, rapid urease test, and immunohistostaining. In addition, serum antibody against H. pylori was tested before the eradication treatment and a (13)C-urea breath test was conducted after treatment. The results of these reference tests were compared with those obtained by HpSA. RESULTS: The sensitivity and specificity of HpSA were 90.4% and 100% before eradication treatment and 57.1% and 100% after the treatment. There was a positive correlation between the optical density of HpSA and the number of H. pylori bacilli evaluated by quantitative culture. CONCLUSIONS: The HpSA test is considered to be an accurate method for the diagnosis of H. pylori infection, with high specificity. However, there may be problems of false negativity if HpSA is used for the early assessment of treatment efficacy. Furthermore, HpSA is suggested to have potential for the quantitative evaluation of H. pylori status in the stomach.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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