Low presence of p53 abnormalities in H. pylori-infected gastric mucosa and in gastric adenocarcinoma.
BACKGROUND: Alterations of the p53 gene and/or its abnormal protein accumulation have been observed in gastric cancer and preneoplastic lesions. Our aim was to assess possible associations between different H. pylori strains and p53 abnormalities in patients with dyspepsia and with gastric cancer. M...
| Publicado en: | Journal of Gastroenterology Vol. 38; no. 1; pp. 28 - 37 |
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| Autores principales: | , , , , , , , , |
| Formato: | diagnostic images research tables/charts Journal Article |
| Publicado: |
Springer Nature
2003
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106876792&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106876792 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09441174 EJ7 jtl: Journal of Gastroenterology issn: 09441174 maglogo: N pubinfo: dt: 2003 vid: 38 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 106876792 2003136036 10.1007/s005350300003 NLM12560919 106876792 ppf: 28 ppct: 9 formats: tig: atl: Low presence of p53 abnormalities in H. pylori-infected gastric mucosa and in gastric adenocarcinoma. aug: au: Berloco P Russo F Cariola F Gentile M Giorgio P Caruso ML Valentini AM Di Matteo G Di Leo A affil: Laboratory of Biochemistry, Scientific Institute for Digestive Diseases, IRCCS 'Saverio de Bellis', 70013 Castellana Grotte (Bari), Italy sug: subj: Adenocarcinoma Pathology Gastric Mucosa Anatomy and Histology Gastric Mucosa Pathology Helicobacter Infections Pathology Adult Aged Aged, 80 and Over Chi Square Test Comparative Studies Endoscopy Female Gene Amplification Immunohistochemistry Italy Male Middle Age Mutation Polymerase Chain Reaction Prevalence Human Adult: 19-44 years Aged: 65+ years Aged, 80 & over Middle Aged: 45-64 years Female Male ab: BACKGROUND: Alterations of the p53 gene and/or its abnormal protein accumulation have been observed in gastric cancer and preneoplastic lesions. Our aim was to assess possible associations between different H. pylori strains and p53 abnormalities in patients with dyspepsia and with gastric cancer. METHODS: Seventy-five dyspeptic patients and 40 patients with gastric adenocarcinoma entered the study. H. pylori status was determined by the rapid urease test, histology, and polymerase chain reaction (PCR) analysis. Overexpression of the p53 protein was evaluated by immunohistochemistry. Detection of p53 mutations was done by direct DNA sequencing. RESULTS: Fifty-four of the 75 (72.0%) dyspeptic patients and 27 of the 40 (67.5%) gastric cancer patients showed H. pylori infection. Cytotoxin-associated gene ( cagA)-positive strains were found in 31 of the 54 (58%) dyspeptic patients and in 25 of the 27 (92.6%) neoplastic patients. As regards vacA, s2 strains showed the highest prevalence among dyspeptic patients (24 of 54 patients; 44.4%), whereas s1 strains were more expressed among cancer patients (23 of 27; 85.2%). Among the dyspeptic patients, 1 patient with duodenal ulcer showed p53 overexpression. Three mutations were identified by DNA sequencing: one in a patient with normal endoscopic findings and two in patients suffering from gastritis. Among the neoplastic patients, 16 subjects (40%) showed p53 overexpression (9 had diffuse-type and 7 intestinal-type cancer). Four mutations (10%) occurred in patients with intestinal-type gastric cancer. No association between p53 abnormalities (overexpression/mutation) and H. pylori infection was found in either group of patients. CONCLUSIONS: These results lead us to hypothesize that H. pylori infection does not affect the p53 pattern in gastric mucosa. Moreover, mutations of the p53 gene do not seem to be a predominant event in gastric carcinogenesis, at least in our populations. pubtype: Academic Journal doctype: diagnostic images research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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