Activation of the complement system by different autologous transfusion devices: an in vitro study.
Background: The aim of the present investigation was to study whether autologous transfusion devices activate the complement system and whether complement-activated blood is more vulnerable to further activation during processing.Study Design and Methods: Forty-eight blood units were randomized to b...
| Publicado en: | Transfusion Vol. 43; no. 3; pp. 395 - 400 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Mar2003
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=106884792&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 106884792 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00411132 1YB jtl: Transfusion issn: 00411132 maglogo: Y pubinfo: dt: Mar2003 vid: 43 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 106884792 NLM12675727 2003153289 10.1046/j.1537-2995.2003.00311.x NLM12675727 106884792 ppf: 395 ppct: 5 formats: tig: atl: Activation of the complement system by different autologous transfusion devices: an in vitro study. aug: au: Tylman M Bengtson JP Bengtsson A Tylman, Maria Bengtson, Jan Peter Bengtsson, Anders affil: Department of Anesthesiology and Intensive Care, Sahlgrenska University Hospital/Ostra, Göteburg, Sweden sug: subj: Blood Transfusion, Autologous Equipment and Supplies Immunity Glycoproteins Blood Centrifugation In Vitro Studies Wilcoxon Signed Rank Test Funding Source Human ab: Background: The aim of the present investigation was to study whether autologous transfusion devices activate the complement system and whether complement-activated blood is more vulnerable to further activation during processing.Study Design and Methods: Forty-eight blood units were randomized to be processed by one of three different salvage systems: Group 1 underwent whole blood filtration (hemofiltration) (n=16); Group 2 underwent continuous processing, saline washing, and centrifugation (CATS, Fresenius AG ) (n=16); and Group 3 underwent saline washing and centrifugation (Cell-Saver, Haemonetics Corp.) (n=16). Eight blood units for each system were activated with cobra venom factor (CVF) at a concentration of 0.2 U per mL whole blood before processing. C activation was studied by determinations of C4d, Bb, C3a, and SC5b-9. Samples were drawn from whole blood, processed blood, and the waste bags.Results: The concentrations of Bb, C3a, and SC5b-9 in whole blood after activation with CVF were significantly elevated compared to blood that was not activated (p < 0.01). Processed blood from hemofiltration contained significantly higher levels of complement-split products than techniques that use washing and centrifugation. The concentrations of SC5b-9 in blood processed by hemofiltration were higher in the experiments with CVF activation (p < 0.05).Conclusion: The tested autologous transfusion systems did not themselves activate the complement system, and complement-activated blood was not more vulnerable to further activation during processing. A blood-salvaging technique that used washing and centrifugation reduced elevated concentrations of complement-split products, whereas hemofiltration did not. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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