Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori: a multicentre study.
Objective: To evaluate the usefulness of a commercial serological enzyme immunoassay (EIA) for detecting salivary immunoglobulin (Ig) G to Helicobacter pylori.Design: Prospective, multicentre study.Setting: The study was conducted at 11 gastroenterology hospital centres throughout Italy.Participants...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 12; no. 10; pp. 1117 - 1121 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
2000 Oct
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=107001981&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 107001981 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: 2000 Oct vid: 12 iid: 10 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 107001981 107001981 NLM11057457 2001017798 10.1097/00042737-200012100-00008 NLM11057457 107001981 ppf: 1117 ppct: 4 formats: tig: atl: Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori: a multicentre study. aug: au: Luzza F Imeneo M Marasco A Crotta S Ierardi E Usai P Virgilio C Nardone G Marchi S Sanna G Perri F Zagari RM Bazzoli F Luzza, F Imeneo, M Marasco, A Crotta, S Ierardi, E Usai, P Virgilio, C affil: Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro Magna Graecia, Catanzaro, Italy sug: subj: Reagent Kits, Diagnostic Immunoglobulins Analysis Immunoassay Saliva Analysis Sensitivity and Specificity Helicobacter Pylori Analysis Predictive Value of Tests Prospective Studies T-Tests Confidence Intervals Adult Middle Age Aged Aged, 80 and Over Female Male Funding Source Human Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Female Male ab: Objective: To evaluate the usefulness of a commercial serological enzyme immunoassay (EIA) for detecting salivary immunoglobulin (Ig) G to Helicobacter pylori.Design: Prospective, multicentre study.Setting: The study was conducted at 11 gastroenterology hospital centres throughout Italy.Participants and Interventions: Two hundred and thirteen dyspeptic patients underwent gastroscopy with antral biopsies. At each centre, two of the following three tests for H. pylori diagnosis were performed: urease quick test, histology, and 13C-urea breath test. Samples of unstimulated saliva and venous blood were collected from each patient. Salivary and serum H. pylori IgG were determined with the EIA Helori-test IgG (Eurospital, Trieste, Italy).Main Outcome Measures: Sensitivity, specificity, positive and negative predictive values, and accuracy of the salivary and serum EIAs.Results: By using a receiver operating characteristic curve, salivary EIA yielded 81% (95% confidence interval 73-87%) sensitivity, 73% (62-83%) specificity, 84% (76-90%) positive predictive value, 69% (58-79%) negative predictive value, and 78% (72-84%) accuracy. At the cut-off suggested by the manufacturer, serum EIA had 90% (84-95%) sensitivity, 78% (67-86%) specificity, 88% (81-93%) positive predictive value, 82% (71-90%) negative predictive value, and 86% (81-91%) accuracy.Conclusion: In this large multicentre study, detection of salivary H. pylori IgG with a commercial serological EIA was a fairly accurate diagnostic method. Data confirm that saliva testing does not compare favourably with serology in the assessment of H. pylori status. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|