A recombinant peptide antigen line immunoassay optimized for the confirmation of Chagas' disease.
Background: The transfusion of contaminated blood has become the major route of transmission for Chagas' disease in Brazil. Current screening tests are insensitive and yield conflicting results, while confirmatory assays do not exist. A line immunoassay (INNO-LIA Chagas Ab [INNO-LIA]) combining rele...
| Publicado en: | Transfusion Vol. 39; no. 7; pp. 711 - 718 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | algorithm research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul99
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=107218154&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 107218154 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00411132 1YB jtl: Transfusion issn: 00411132 maglogo: Y pubinfo: dt: Jul99 vid: 39 iid: 7 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 107218154 107218154 NLM10413278 1999070154 NLM10413278 107218154 ppf: 711 ppct: 7 formats: tig: atl: A recombinant peptide antigen line immunoassay optimized for the confirmation of Chagas' disease. aug: au: Oelemann WMR Vanderborght BOM Verissimo Da Costa GC Teixeira MGM Borges-Pereira J De Castro JAF Coura JR Stoops E Hulstaert F Zrein M Peralta JM Oelemann, W M Vanderborght, B O Verissimo Da Costa, G C Teixeira, M G Borges-Pereira, J De Castro, J A Coura, J R Stoops, E Hulstaert, F affil: Institute of Microbiology and the Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Brazil sug: subj: Trypanosomiasis Diagnosis Immunoassay Blood Donors Retrospective Design Technology, Medical Sensitivity and Specificity Human ab: Background: The transfusion of contaminated blood has become the major route of transmission for Chagas' disease in Brazil. Current screening tests are insensitive and yield conflicting results, while confirmatory assays do not exist. A line immunoassay (INNO-LIA Chagas Ab [INNO-LIA]) combining relevant, immunodominant recombinant and synthetic antigens on a single nylon membrane strip was evaluated for the serologic confirmation of Chagas' disease.Study Design and Methods: Sera from 1062 patients and healthy residents of four Brazilian regions endemic for Chagas' disease were used for test optimization. The established confirmation algorithm was evaluated with an independent set of positive (n = 75) and negative (n = 148) samples.Results: In the optimization phase, without an established comparative gold standard, the results with the INNO-LIA were compared with those obtained in four other screening assays. In the validation phase, the INNO-LIA showed a sensitivity of 100 percent (95% CI, 95.21-100) and a specificity of 99.32 percent (95% CI, 96.29-99.98) for well-characterized sera. Moreover, its specificity reached 100 percent with a set of 40 sera obtained from patients with documented leishmaniasis. The interpretation criteria defined in this study indicated that the INNO-LIA accurately detected the presence of antibodies to various specific antigens of Trypanosoma cruzi.Conclusion: The INNO-LIA Chagas Ab assay may become the first commercial assay to reliably confirm the presence of antibodies to T. cruzi. pubtype: Academic Journal doctype: algorithm research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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