Automation of an interferon-γ release assay and comparison to the tuberculin skin test for screening basic military trainees for Mycobacterium tuberculosis infection.
We automated portions of the QuantiFERON-TB Gold In-Tube test (QFT-GIT) and assessed its quality when performed concurrently with the tuberculin skin test (TST) among U.S. Air Force basic military trainees (BMTs). The volume of blood collected for QFT-GIT was monitored. At least one of the three tub...
| Publicado en: | Military Medicine Vol. 179; no. 3; pp. 333 - 342 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
Mar2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=107790374&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 107790374 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00264075 4DV jtl: Military Medicine issn: 00264075 maglogo: N pubinfo: dt: Mar2014 vid: 179 iid: 3 pid: 622 pub: Oxford University Press / USA artinfo: ui: 107790374 94856438 NLM24594471 2012499360 10.7205/MILMED-D-13-00364 NLM24594471 107790374 ppf: 333 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Automation of an interferon-γ release assay and comparison to the tuberculin skin test for screening basic military trainees for Mycobacterium tuberculosis infection. aug: au: Goodwin, Donald J Mazurek, Gerald H Campbell, Brandon H Bohanon, Jamaria West, Kevin B Bell, James J Powell, Richard Toney, Sean Morris, John A Yamane, Grover K Sjoberg, Paul A affil: Epidemiology Consult Services, U.S. Air Force School of Aerospace Medicine, 2510 Fifth Street, Building 840, W318K, Wright-Patterson AFB, OH 45433-7913. sug: subj: Antibodies, Bacterial Analysis Automation Interferon-gamma Release Tests Methods Military Personnel Mycobacterium Tuberculosis Immunology Tuberculin Test Methods Tuberculosis Diagnosis Adult Female Prospective Studies Human Incidence Male Prevalence Retrospective Design Tuberculosis Epidemiology Tuberculosis Microbiology United States Young Adult Adult: 19-44 years Female Male ab: We automated portions of the QuantiFERON-TB Gold In-Tube test (QFT-GIT) and assessed its quality when performed concurrently with the tuberculin skin test (TST) among U.S. Air Force basic military trainees (BMTs). The volume of blood collected for QFT-GIT was monitored. At least one of the three tubes required for QFT-GIT had blood volume outside the recommended 0.8- to 1.2-mL range for 688 (29.0%) of 2,373 subjects who had their blood collected. Of the 2,124 subjects who had TST and QFT-GIT completed, TST was positive for 0.6%; QFT-GIT was positive for 0.3% and indeterminate for 2.0%. Among 2,081 subjects with completed TST and determinate QFT-GIT results, overall agreement was 99.5% but positive agreement was 5.6%. Specificity among the 1,546 low-risk BMTs was identical (99.7%). Indeterminate QFT-GIT results were 2.7 times more likely when mitogen tubes contained >1.2 mL blood than when containing 0.8- to 1.2-mL blood. Automation can facilitate QFT-GIT completion, especially if the recommended volume of blood is collected. Mycobacterium tuberculosis infection prevalence among BMTs based on TST and QFT-GIT is similar and low. Selectively testing those with significant risk may be more appropriate than universal testing of all recruits. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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