Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 survivin transcripts and evaluation in breast cancer tissues.
BACKGROUND: Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in bre...
| Publicado en: | Clinical Breast Cancer Vol. 14; no. 2; pp. 122 - 132 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Apr2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=107894423&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 107894423 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15268209 G4D jtl: Clinical Breast Cancer issn: 15268209 maglogo: N pubinfo: dt: Apr2014 vid: 14 iid: 2 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 107894423 2012512636 10.1016/j.clbc.2013.10.012 NLM24325949 107894423 ppf: 122 ppct: 10 formats: tig: atl: Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 survivin transcripts and evaluation in breast cancer tissues. aug: au: Pavlidou, Anastasia Kroupis, Christos Goutas, Nikolaos Dalamaga, Maria Dimas, Kleanthi affil: Department of Clinical Biochemistry, University of Athens Medical School, Attikon University Hospital, Haidari, Greece. sug: subj: Breast Neoplasms Genes Proteins RNA Breast Neoplasms Pathology Female Human Prognosis Neoplasm Invasiveness Survival Female ab: BACKGROUND: Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in breast cancer. METHODS: Real-time quantitative polymerase chain reaction (qPCR) was performed on the cDNA with a reverse primer specific for each splice variant and a pair of common hybridization probes. RESULTS: The expression of wild-type survivin was significantly correlated with survivin-2b, survivin-[Delta]Ex3, and the ratio of survivin-[Delta]Ex3 to wild-type survivin (P < .001). The ratio of survivin-2b to wild-type survivin was strongly associated with the ratio of survivin-[Delta]Ex3 to wild-type survivin (P < .001). There was a strong positive association between the grade of the tumor and survivin-2b mRNA, survivin-[Delta]Ex3 mRNA, and the ratio of survivin-[Delta]Ex3 to wild-type survivin mRNA (P < .05). The ratio of survivin-2b to wild-type survivin was significantly associated with the presence of estrogen receptors (P = .05). CONCLUSION: Our validated data suggest that survivin isoforms may be related to clinicopathological features and could be used as molecular prognostic tools or as new therapy targets. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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