Volumetric optical frequency domain imaging of pulmonary pathology with precise correlation to histopathology.
Background: Lung cancer is the leading cause of cancer-related mortality. Radiology and bronchoscopy techniques do not have the necessary resolution to evaluate lung lesions on the microscopic scale, which is critical for diagnosis. Bronchial biopsy specimens can be limited by sampling error and sma...
| Publicado en: | CHEST Vol. 143; no. 1; pp. 64 - 75 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American College of Chest Physicians
Jan2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=108028879&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 108028879 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00123692 1G4 jtl: CHEST issn: 00123692 maglogo: N pubinfo: dt: Jan2013 vid: 143 iid: 1 pid: 2556 pub: American College of Chest Physicians place: Glenview, Illinois artinfo: ui: 108028879 NLM22459781 2011932353 10.1378/chest.11-2797 NLM22459781 PMC3537541 108028879 ppf: 64 ppct: 11 formats: tig: atl: Volumetric optical frequency domain imaging of pulmonary pathology with precise correlation to histopathology. aug: au: Hariri LP Applegate MB Mino-Kenudson M Mark EJ Medoff BD Luster AD Bouma BE Tearney GJ Suter MJ Hariri, Lida P Applegate, Matthew B Mino-Kenudson, Mari Mark, Eugene J Medoff, Benjamin D Luster, Andrew D Bouma, Brett E Tearney, Guillermo J Suter, Melissa J affil: Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA sug: subj: Adenocarcinoma Pathology Bronchoscopy Methods Carcinoma, Squamous Cell Pathology Lung Diseases Pathology Lung Neoplasms Pathology Tomography Methods Neoplasms, Cystic, Mucinous, and Serous Pathology Carcinoma, Adenoid Cystic Pathology Hamartoma Pathology Human Pulmonary Fibrosis Pathology ab: Background: Lung cancer is the leading cause of cancer-related mortality. Radiology and bronchoscopy techniques do not have the necessary resolution to evaluate lung lesions on the microscopic scale, which is critical for diagnosis. Bronchial biopsy specimens can be limited by sampling error and small size. Optical frequency domain imaging (OFDI) provides volumetric views of tissue microstructure at near-histologic resolution and may be useful for evaluating pulmonary lesions to increase diagnostic accuracy. Bronchoscopic OFDI has been evaluated in vivo, but a lack of correlated histopathology has limited the ability to develop accurate image interpretation criteria.Methods: We performed OFDI through two approaches (airway-centered and parenchymal imaging) in 22 ex vivo lung specimens, using tissue dye to precisely correlate imaging and histology.Results: OFDI of normal airway allowed visualization of epithelium, lamina propria, cartilage, and alveolar attachments. Carcinomas exhibited architectural disarray, loss of normal airway and alveolar structure, and rapid light attenuation. Squamous cell carcinomas showed nested architecture. Atypical glandular formation was appreciated in adenocarcinomas, and uniform trabecular gland formation was seen in salivary gland carcinomas. Mucinous adenocarcinomas showed alveolar wall thickening with intraalveolar mucin. Interstitial fibrosis was visualized as signal-dense tissue, with an interstitial distribution in mild interstitial fibrotic disease and a diffuse subpleural pattern with cystic space formation in usual interstitial pneumonitis.Conclusions: To our knowledge, this study is the first demonstration of volumetric OFDI with precise correlation to histopathology in lung pathology. We anticipate that OFDI may play a role in assessing airway and parenchymal pathology, providing fresh insights into the volumetric features of pulmonary disease. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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