Leishmania detection in sand flies using a field-deployable real-time analytic system.
We describe here the development and evaluation of advanced vector surveillance analytic technologies for real-time leishmaniasis risk assessment. Leishmania genus and visceral leishmaniasis causative agent--specific dual fluorogenic-probe hydrolysis (TaqMan), thermally stable (freeze-dried) polymer...
| Published in: | Military Medicine Vol. 177; no. 4; pp. 460 - 467 |
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| Main Authors: | , , , , , , , , , |
| Format: | research Journal Article |
| Published: |
Oxford University Press / USA
Apr2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=108114478&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 108114478 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00264075 4DV jtl: Military Medicine issn: 00264075 maglogo: N pubinfo: dt: Apr2012 vid: 177 iid: 4 pid: 622 pub: Oxford University Press / USA artinfo: ui: 108114478 108114478 NLM22594139 2011555608 10.7205/milmed-d-11-00206 NLM22594139 108114478 ppf: 460 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Leishmania detection in sand flies using a field-deployable real-time analytic system. aug: au: McAvin JC Swanson KI Chan AS Quintana M Coleman RE McAvin, James C Swanson, Katherine I Chan, Adeline S T Quintana, Miguel Coleman, Russell E affil: Division of Entomology, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA sug: subj: Computer Systems Leishmania Leishmaniasis Insects Animals DNA Human Iraq Military Medicine Polymerase Chain Reaction Population Surveillance Predictive Value of Tests Risk Assessment Sensitivity and Specificity ab: We describe here the development and evaluation of advanced vector surveillance analytic technologies for real-time leishmaniasis risk assessment. Leishmania genus and visceral leishmaniasis causative agent--specific dual fluorogenic-probe hydrolysis (TaqMan), thermally stable (freeze-dried) polymerase chain reaction assays were developed using field-durable analytic instrumentation. In laboratory testing with a panel of diverse Leishmania species from culture and infected sand flies, the sensitivity and specificity of both assays were 100% concordant with DNA sequencing. In specificity testing with Leishmania genetic near neighbors, clinically significant organisms, and human genomic DNA, no detectable fluorescence above background was observed. Field evaluation was conducted in southern Iraq using wild sand flies. In field testing, Leishmania genus assay was 100% sensitive and 96% specific with a single false-positive result. The visceral leishmaniasis genotype assay was 100% sensitive and 100% specific compared to DNA sequencing. Thermally stable polymerase chain reaction assays vastly simplified transportation and storage. Assay preparation and analysis required less than 2 hours. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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