[Delta]Np63 (p40) and thyroid transcription factor-1 immunoreactivity on small biopsies or cellblocks for typing non-small cell lung cancer: a novel two-hit, sparing-material approach.
INTRODUCTION: Diagnosing non-small cell lung cancer on biopsy/cellblock samples by morphology may be demanding. As sparing material for molecular testing is mandatory, a minimalist immunohistochemistry (IHC)-based diagnostic approach is warranted by means of novel, reliable, and easy-to-assess bioma...
| Publicado en: | Journal of Thoracic Oncology Vol. 7; no. 2; pp. 281 - 291 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Elsevier B.V.
2012 Feb
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=108163822&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 108163822 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15560864 4Z2B jtl: Journal of Thoracic Oncology issn: 15560864 maglogo: N pubinfo: dt: 2012 Feb vid: 7 iid: 2 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 108163822 2011473964 NLM22071786 108163822 ppf: 281 ppct: 10 formats: tig: atl: [Delta]Np63 (p40) and thyroid transcription factor-1 immunoreactivity on small biopsies or cellblocks for typing non-small cell lung cancer: a novel two-hit, sparing-material approach. aug: au: Pelosi G Fabbri A Bianchi F Maisonneuve P Rossi G Barbareschi M Graziano P Cavazza A Rekhtman N Pastorino U Scanagatta P Papotti M affil: Department of Pathology and Laboratory Medicine,Fondazione IRCCS National Cancer Institute, Milan, Italy. giuseppe.pelosi@istitutotumori.mi.it sug: subj: Carcinoma, Non-Small-Cell Lung Classification Carcinoma, Non-Small-Cell Lung Metabolism Proteins Metabolism Lung Neoplasms Classification Lung Neoplasms Metabolism Adenocarcinoma Metabolism Adult Aged Neoplasms, Squamous Cell Metabolism Carcinoma, Squamous Cell Metabolism Female Immunoenzyme Techniques Male Middle Age Proteins Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: INTRODUCTION: Diagnosing non-small cell lung cancer on biopsy/cellblock samples by morphology may be demanding. As sparing material for molecular testing is mandatory, a minimalist immunohistochemistry (IHC)-based diagnostic approach is warranted by means of novel, reliable, and easy-to-assess biomarkers. METHODS: Forty-six consecutive biopsy/cellblock samples and the corresponding resection specimens (as the gold standard for morphology and IHC) from 30 adenocarcinomas (AD), 10 squamous carcinomas (SQC), 5 adenosquamous carcinomas (ADSQC), and 1 sarcomatoid carcinoma (SC) were IHC-evaluated for p40 [corresponding to nontransactivating [Delta]Np63 isoforms] and thyroid transcription factor-1 (TTF1) by semiquantitative assessment. For p40, also immunodecoration intensity was taken into account and dichotomized as strong or low. RESULTS: Nonrandom and overlapping distributions of the relevant markers were found in biopsy/cellblock and surgical specimens, which closely correlated with each other and the diverse tumor categories, with no differences in area under curve-receiver-operating-characteristic curves for each marker between any two samples, including p40 and p63. Diagnostic combinations were p40-/TTF1+ or TTF1- for AD (where p40 was negative, apart from 5/30 AD showing at the best 1-2% tumor cells with low intensity); p40+/TTF1- (p40 strong and by far higher than 50%) for SQC; and p40+/TTF1+ or p40+/TTF1- (p40 strong and less than 50%) for ADSQC. The single SC case was p40-/TTF1-, suggesting glandular lineage. Practically, 41/46 (89%) tumors were correctly classified by IHC on small samples, including 30 AD, 10 SQC, 1/5 ADSQC, and no SC. Underdiagnosis of ADSQC was actually because of sampling error of biopsies/cellblocks rather than insufficient biomarker robustness, whereas underdiagnosis of SC was really because of the failure of either marker to highlight epithelial-mesenchymal transition. CONCLUSIONS: This minimalist IHC-based model of p40 and TTF1 on biopsy/cellblock samples was effective to correctly subtype most cases of lung cancer. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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