Detection of Epistatic and Gene-Environment Interactions Underlying Three Quality Traits in Rice Using High-Throughput Genome-Wide Data.

With development of sequencing technology, dense single nucleotide polymorphisms (SNPs) have been available, enabling uncovering genetic architecture of complex traits by genome-wide association study (GWAS). However, the current GWAS strategy usually ignores epistatic and gene-environment interacti...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 8
Autores principales: Xu, Haiming, Jiang, Beibei, Cao, Yujie, Zhang, Yingxin, Zhan, Xiaodeng, Shen, Xihong, Cheng, Shihua, Lou, Xiangyang, Cao, Liyong
Formato: research tables/charts Journal Article
Publicado: Wiley-Blackwell 8/4/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 8/4/2015
      vid: 2015
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2015/135782
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        atl: Detection of Epistatic and Gene-Environment Interactions Underlying Three Quality Traits in Rice Using High-Throughput Genome-Wide Data.
      aug:
        au:
          Xu, Haiming
          Jiang, Beibei
          Cao, Yujie
          Zhang, Yingxin
          Zhan, Xiaodeng
          Shen, Xihong
          Cheng, Shihua
          Lou, Xiangyang
          Cao, Liyong
        affil: Institute of Crop Science and Institute of Bioinformatics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
      sug:
        subj:
          Technology, Pharmaceutical Methods
          Genes
          Environment
          Rice Analysis
          Polymorphism, Genetic
          Simulations
          Genotype
          Descriptive Statistics
          Data Analysis Software
          Funding Source
          China
      ab: With development of sequencing technology, dense single nucleotide polymorphisms (SNPs) have been available, enabling uncovering genetic architecture of complex traits by genome-wide association study (GWAS). However, the current GWAS strategy usually ignores epistatic and gene-environment interactions due to absence of appropriate methodology and heavy computational burden. This study proposed a new GWAS strategy by combining the graphics processing unit- (GPU-) based generalized multifactor dimensionality reduction (GMDR) algorithm with mixed linear model approach. The reliability and efficiency of the analytical methods were verified through Monte Carlo simulations, suggesting that a population size of nearly 150 recombinant inbred lines (RILs) had a reasonable resolution for the scenarios considered. Further, a GWAS was conducted with the above two-step strategy to investigate the additive, epistatic, and gene-environment associations between 701,867 SNPs and three important quality traits, gelatinization temperature, amylose content, and gel consistency, in a RIL population with 138 individuals derived from super-hybrid rice Xieyou9308 in two environments. Four significant SNPs were identified with additive, epistatic, and gene-environment interaction effects. Our study showed that the mixed linear model approach combining with the GPU-based GMDR algorithm is a feasible strategy for implementing GWAS to uncover genetic architecture of crop complex traits.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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