Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru.
Background Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii . Aims To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neo...
| Published in: | Revista Iberoamericana de Micologia Vol. 32; no. 2; pp. 88 - 93 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Article |
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Elsevier B.V.
Apr-Jun2015
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=109218927&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 109218927 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 11301406 PVP jtl: Revista Iberoamericana de Micologia issn: 11301406 maglogo: N pubinfo: dt: Apr-Jun2015 vid: 32 iid: 2 pid: 467 pub: Elsevier B.V. artinfo: ui: 109218927 10.1016/j.riam.2014.01.005 ppf: 88 ppct: 5 formats: fmt: @attributes: type: T tig: atl: Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru. aug: au: Bejar, Vilma Tello, Mercedes García, Ruth Guevara, José M. Gonzales, Sofia Vergaray, German Valencia, Esther Abanto, Enma Ortega-Loayza, Alex G. Hagen, Ferry Gutierrez, Ericson L. affil: Instituto de Medicina Tropical "Daniel Alcides Carrión", Facultad de Medicina, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru Facultad de Ciencias Biológicas, UNMSM, Lima, Peru Department of Dermatology, Virginia Commonwealth University, Richmond, VA, USA CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands Unidad de Análisis y Generación de Evidencia en Salud Pública, Instituto Nacional de Salud, Lima, Peru Facultad de Medicina, Universidad de San Martín de Porres, Lima, Peru su: Cryptococcus neoformans Molecular structure Microbial sensitivity tests Antifungal agents Serotypes Genotypes sug: subj: Cryptococcus neoformans Molecular structure Microbial sensitivity tests Antifungal agents Serotypes Genotypes keyword: Genes del tipo sexual de los hongos Genotipo Genotype Mating-type Serotipificación Serotyping Cryptococcus neoformans Genes del tipo sexual de los hongos Genotipo Genotype Mating-type Serotipificación Serotyping ab: Background Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii . Aims To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results Using conventional PCR, 27 (84.4%) of the isolates were identified as C. neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (6.3%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C. neoformans are sensitive to these antifungal compounds. Conclusions In this study we observed that C. neoformans var. grubii (AFLP1 and AFLP1B) and C. neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature. Resumen Antecedentes La criptococosis es una infección fúngica de distribución mundial, causada principalmente por Cryptococcus neoformans y Cryptococcus gattii . Objetivos Determinar el tipo de apareamiento, los serotipos, los genotipos y la sensibilidad antifúngica de las cepas de C. neoformans provenientes de Lima Perú. Métodos Se analizaron 32 cepas de Cryptococcus spp. del Instituto de Medicina Tropical de la Universidad Nacional Mayor de San Marcos de Lima, Perú. Veinticuatro cepas provenían de pacientes y 8 cepas fueron obtenidas de muestras ambientales. Resultados Mediante la técnica de reacción en cadena de la polimerasa se determinó que 27 (84,4%) aislamientos eran C. neoformans var. grubii , serotipo A, con el tipo de apareamiento alfa. Asimismo, empleando la técnica AFLP, se determinó que 16 (50%) cepas de C. neoformans eran del genotipo AFLP1 y 13 (40,6%) del genotipo AFLP1B; además, 2 (6,3%) eran del genotipo AFLP2 ( C. neoformans var. neoformans ) y 1 (3,1%) resultó ser un híbrido entre ambas variedades (AFLP3). Los perfiles de sensibilidad antifúngica para anfotericina B, fluconazol y voriconazol mostraron que las 32 cepas de C. neoformans eran sensibles al panel de antifúngicos. Conclusiones En el presente estudio observamos que C. neoformans var. grubii (AFLP1 y AFLP1B) y C. neoformans var. neoformans (AFLP2) fueron las variedades encontradas. Además, todas las cepas resultaron sensibles al panel de antifúngicos, en concordancia con la literatura internacional. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Iberoamericana de Micologia holder: Elsevier B.V. dt: @attributes: year: 2015 holdings: @attributes: islocal: N |
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